D7SSD8 · MJE1_VITVI
- ProteinMethyl jasmonate esterase 1
- GeneMJE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids261 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methylesterase that catalyzes the hydrolysis of methyl jasmonate (MeJA) into jasmonate (JA) (PubMed:26934101).
Can also use methyl salicylate (MeSA) as substrate with a lower efficiency (PubMed:26934101).
Can also use methyl salicylate (MeSA) as substrate with a lower efficiency (PubMed:26934101).
Catalytic activity
- H2O + methyl --jasmonate = H+ + jasmonate + methanolThis reaction proceeds in the forward direction.
- H2O + methyl salicylate = H+ + methanol + salicylateThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
92.9 μM | methyl jasmonate |
Pathway
Plant hormone biosynthesis.
Lipid metabolism; oxylipin biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 82 | Acyl-ester intermediate | ||||
Sequence: S | ||||||
Active site | 211 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 239 | Charge relay system | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | methyl indole-3-acetate esterase activity | |
Molecular Function | methyl jasmonate esterase activity | |
Molecular Function | methyl salicylate esterase activity | |
Biological Process | jasmonic acid metabolic process | |
Biological Process | oxylipin biosynthetic process | |
Biological Process | response to cold | |
Biological Process | response to UV-B | |
Biological Process | salicylic acid metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMethyl jasmonate esterase 1
- EC number
- Short namesVvMJE1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > Vitales > Vitaceae > Viteae > Vitis
Accessions
- Primary accessionD7SSD8
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454242 | 1-261 | Methyl jasmonate esterase 1 | |||
Sequence: MEKRERHFVLVHGACHGAWCWYKVTTFLRSAGHKVTALDLAAAGANGKRLDELNSISDYHEPLMKFMTSLVAGEKVILVAHSLGGVSVSVAMERFPQKISVAVFVSAYMPGPDFNLSTVYQELHQRRQGASKDTQYTFDRGSNNPPTSIIFSPEDLAAKLYQLSPPEDLTLATTLMRPTKLFRGENLLKETTVTREKYGTVRRVYIVCDKDNILKEDFQRWMIKNNPSDEVKVIMGSDHMPMFSKPLDLCAYLQEIVESYS |
Proteomic databases
Expression
Induction
By cold and UV-B.
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-251 | AB hydrolase-1 | ||||
Sequence: FVLVHGACHGAWCWYKVTTFLRSAGHKVTALDLAAAGANGKRLDELNSISDYHEPLMKFMTSLVAGEKVILVAHSLGGVSVSVAMERFPQKISVAVFVSAYMPGPDFNLSTVYQELHQRRQGASKDTQYTFDRGSNNPPTSIIFSPEDLAAKLYQLSPPEDLTLATTLMRPTKLFRGENLLKETTVTREKYGTVRRVYIVCDKDNILKEDFQRWMIKNNPSDEVKVIMGSDHMPMFSKPLDLCA |
Sequence similarities
Belongs to the AB hydrolase superfamily. Methylesterase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length261
- Mass (Da)29,486
- Last updated2010-08-10 v1
- Checksum736434B97B8E84C6
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 202 | in Ref. 3; RVW57636 | ||||
Sequence: R → H |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FN594975 EMBL· GenBank· DDBJ | CBI18570.3 EMBL· GenBank· DDBJ | Genomic DNA | ||
QGNW01001049 EMBL· GenBank· DDBJ | RVW57636.1 EMBL· GenBank· DDBJ | Genomic DNA |