D7REY5 · CDHC_PSEU3
- ProteinCaffeine dehydrogenase subunit gamma
- GenecdhC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids167 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the caffeine dehydrogenase complex that catalyzes the hydrolytical oxidation of 1,3,7-trimethylxanthine (caffeine) by incorporation of an oxygen atom originating from a water molecule into position C-8 to produce 1,3,7-trimethyluric acid (TMU). Coenzyme Q0 (ubiquinone-0) is the preferred electron acceptor and, to a lesser extent, coenzyme Q2 (ubiquinone-2) can also be used, but oxygen and NAD(P)+ cannot. Is involved in a caffeine degradation pathway that allows Pseudomonas sp. strain CBB1 to grow on caffeine as the sole carbon and nitrogen source. Is also active with theobromine as substrate, but shows a very poor activity with theophylline and is not active with xanthine, 3-methylxanthine, 7-methylxanthine, TMU, and 3,7-dimethylurate.
Catalytic activity
- caffeine + a ubiquinone + H2O = 1,3,7-trimethylurate + a ubiquinolThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.7 μM | caffeine | 7 | 35 |
kcat is 10 min-1 with caffeine as substrate.
pH Dependence
Optimum pH is 7.0.
Temperature Dependence
The caffeine dehydrogenase activity increases linearly from 25 to at least 66 degrees Celsius. An incubation of 30 minutes at 70 degrees Celsius drastically reduces the enzyme activity to 11% of the initial value, and 77% of the activity remains after 30 minutes of incubation at 42 degrees Celsius.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 42 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 47 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 50 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 62 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | caffeine oxidase activity | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCaffeine dehydrogenase subunit gamma
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionD7REY5
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000418589 | 1-167 | Caffeine dehydrogenase subunit gamma | ||
Interaction
Subunit
Heterotrimer composed of an alpha (CdhA), a beta (CdhB) and a gamma (CdhC) subunit.
Structure
Sequence
- Sequence statusComplete
- Length167
- Mass (Da)18,034
- Last updated2010-08-10 v1
- Checksum090A17DBCA952B1A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HM053473 EMBL· GenBank· DDBJ | ADH15881.1 EMBL· GenBank· DDBJ | Genomic DNA |