D7REY5 · CDHC_PSEU3

Function

function

Component of the caffeine dehydrogenase complex that catalyzes the hydrolytical oxidation of 1,3,7-trimethylxanthine (caffeine) by incorporation of an oxygen atom originating from a water molecule into position C-8 to produce 1,3,7-trimethyluric acid (TMU). Coenzyme Q0 (ubiquinone-0) is the preferred electron acceptor and, to a lesser extent, coenzyme Q2 (ubiquinone-2) can also be used, but oxygen and NAD(P)+ cannot. Is involved in a caffeine degradation pathway that allows Pseudomonas sp. strain CBB1 to grow on caffeine as the sole carbon and nitrogen source. Is also active with theobromine as substrate, but shows a very poor activity with theophylline and is not active with xanthine, 3-methylxanthine, 7-methylxanthine, TMU, and 3,7-dimethylurate.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.7 μMcaffeine735
kcat is 10 min-1 with caffeine as substrate.

pH Dependence

Optimum pH is 7.0.

Temperature Dependence

The caffeine dehydrogenase activity increases linearly from 25 to at least 66 degrees Celsius. An incubation of 30 minutes at 70 degrees Celsius drastically reduces the enzyme activity to 11% of the initial value, and 77% of the activity remains after 30 minutes of incubation at 42 degrees Celsius.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site42[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site47[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site50[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site62[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functioncaffeine oxidase activity
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Caffeine dehydrogenase subunit gamma
  • EC number
  • Alternative names
    • Caffeine dehydrogenase small subunit

Gene names

    • Name
      cdhC

Organism names

Accessions

  • Primary accession
    D7REY5

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004185891-167Caffeine dehydrogenase subunit gamma

Interaction

Subunit

Heterotrimer composed of an alpha (CdhA), a beta (CdhB) and a gamma (CdhC) subunit.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-802Fe-2S ferredoxin-type

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    167
  • Mass (Da)
    18,034
  • Last updated
    2010-08-10 v1
  • Checksum
    090A17DBCA952B1A
MSSHVISLTVNGQAIERKVDSRTLLADFLRDELRLTGTHVGCEHGVCGACTIQFDGEPARSCLMLAVQAEGHSIRTVEALAVDGCLGALQQAFHEKHGLQCGFCTPGLLMTLDYALTADLHIDFSSDKEIRELISGNLCRCTGYQNIINAIKSVSPTTEIAKSEELV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HM053473
EMBL· GenBank· DDBJ
ADH15881.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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