D7PVW6 · D7PVW6_9NEOB
- ProteinV(D)J recombination-activating protein 1
- GeneRAG1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids177 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | endonuclease activity | |
Molecular Function | histone binding | |
Molecular Function | protein homodimerization activity | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | B cell differentiation | |
Biological Process | chromatin organization | |
Biological Process | T cell differentiation in thymus | |
Biological Process | V(D)J recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameV(D)J recombination-activating protein 1
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Neobatrachia > Hyloidea > Hylidae > Hylinae > Hylini > Hyla
Accessions
- Primary accessionD7PVW6
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusFragment
- Length177
- Mass (Da)19,989
- Last updated2010-08-10 v1
- ChecksumC30D2A9727C7F42C
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: V | ||||||
Non-terminal residue | 177 | |||||
Sequence: S |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GU989041 EMBL· GenBank· DDBJ | ADI60067.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
GU989051 EMBL· GenBank· DDBJ | ADI60077.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
GU989053 EMBL· GenBank· DDBJ | ADI60079.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
GU989061 EMBL· GenBank· DDBJ | ADI60087.1 EMBL· GenBank· DDBJ | Genomic DNA |