D7EZJ3 · XYNB_STRSQ

  • Protein
    Endo-1,4-beta-xylanase B
  • Gene
    xynBS9
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Endo-acting xylanase which specifically cleaves internal linkages on the xylan backbone, releasing xylooligosaccharides. Is able to hydrolyze oat spelt xylan and birchwood xylan, releasing xylobiose and xylotriose as the major products.

Catalytic activity

  • Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
    EC:3.2.1.8 (UniProtKB | ENZYME | Rhea)

Activity regulation

Is inhibited by Ag+ and Hg2+ in vitro. EDTA and other metal cations such as Na+, K+, Ca2+, Cu2+, Mg2+, Fe3+, and Zn2+ have no effect on catalytic activity.

Kinetics

Vmax pH TEMPERATURE[C] NOTES EVIDENCE
3741 μmol/min/mg6.560with oat spelt xylan as substrate
2320 μmol/min/mg6.560with birchwood xylan as substrate

pH Dependence

Optimum pH is 6-7.

Temperature Dependence

Optimum temperature is 60 degrees Celsius.

Pathway

Glycan degradation; xylan degradation.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site138Nucleophile
Active site227Proton donor

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionendo-1,4-beta-xylanase activity
Molecular Functionpolysaccharide binding
Biological Processxylan catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM2Carbohydrate-Binding Module Family 2
    • GH11Glycoside Hydrolase Family 11

Names & Taxonomy

Protein names

  • Recommended name
    Endo-1,4-beta-xylanase B
  • EC number
  • Short names
    Xylanase B

Gene names

    • Name
      xynBS9

Organism names

  • Taxonomic identifier
  • Strain
    • S9
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    D7EZJ3

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-52
ChainPRO_000042441753-340Endo-1,4-beta-xylanase B

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-20Disordered
Domain54-239GH11
Domain248-340CBM2

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    340
  • Mass (Da)
    36,245
  • Last updated
    2010-08-10 v1
  • Checksum
    84EBAF44059CCCB3
MHDAPAQRKRRRPGRIGPLPRSSRFARLKLLIASACAALLATLALPPGAAHAQTVTSNQTGNHNGYFYSFWTDAPGTVSATMGSGGNYSTSWRNTGNFVIGKGWSTGGRRTVTYSGSFNPSGNAYLTLYGWSRNPLVEYYIVDNWGTYRPTGTFKGTVTTDGGTYDIYQTTRYNAPSIEGNKTFNQYWSVRQQKRTGGTITTGNHFDAWARAGMQLGSHDYMIMATEGYQSSGSSNITVGGTSGGGGGGGGGGGCTATLSAGERWDDRYNLNVSVSGSSNWTVTMNVPSPATILSTWNITATWPSSQVLVARPNGSGNNFGVTIKHNGNWTWPTVSCSTG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU660499
EMBL· GenBank· DDBJ
ACF57947.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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