D6Z6J4 · D6Z6J4_DESAT

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site59-63(6S)-NADPHX (UniProtKB | ChEBI)
Binding site60K+ (UniProtKB | ChEBI)
Binding site131K+ (UniProtKB | ChEBI)
Binding site135-141(6S)-NADPHX (UniProtKB | ChEBI)
Binding site164(6S)-NADPHX (UniProtKB | ChEBI)
Binding site167K+ (UniProtKB | ChEBI)
Binding site266(6S)-NADPHX (UniProtKB | ChEBI)
Binding site336(6S)-NADPHX (UniProtKB | ChEBI)
Binding site390(6S)-NADPHX (UniProtKB | ChEBI)
Binding site427-431AMP (UniProtKB | ChEBI)
Binding site456AMP (UniProtKB | ChEBI)
Binding site457(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular Functionkinase activity
Molecular Functionmetal ion binding
Molecular FunctionNADHX epimerase activity
Molecular FunctionNADPHX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process
Biological Processphosphorylation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional NAD(P)H-hydrate repair enzyme
  • Alternative names
    • Nicotinamide nucleotide repair protein

Including 2 domains:

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase
  • Recommended name
    NAD(P)H-hydrate epimerase
  • EC number

Gene names

    • Name
      nnrE
    • Synonyms
      nnrD
    • Ordered locus names
      DaAHT2_0242

Organism names

Accessions

  • Primary accession
    D6Z6J4

Proteomes

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain9-221YjeF N-terminal
Domain231-517YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    520
  • Mass (Da)
    53,627
  • Last updated
    2010-08-10 v1
  • Checksum
    6F6E039F2B7F392D
MKLASAEQMRAGDRLAMEIFGIPGPVLMENAGRAVVEVMAHRYGLAGEGEIAVLAGPGNNGGDALVIARHLQQLGARPWVVLLVEPERLQGDAALNLQILRQLPVSLEVVTEVEQWQALQDRLQASRLAVDGIFGTGLSRELSGRFQVAVELLNRFPGPVIAVDAPSGLDSDHGRVLGAAVRADCTVTFALAKPGLVTFPGRAWAGTVEVVDIGIPPAVYDRLAPFKELLTAAELAPWLPRRRPVDHKGSFGHLLLLAGSRGMTGAALLAARGALRSGVGLVSACVPRDLLVIFATAWPELMTVALPNSPLAAGAEDLATVQAALAGKSALVLGPGLGTAPATAELVRQLYRTPGELQIPQVLDADALNIMAAAPDLLTEPPGPRVLTPHPGEMARLLGMATAEVQENRWQAAAELAGRHGVWVVLKGAGTVIAAPDGQLAVSPTGNPGMAAGGMGDVLAGLIGGLLAQGMGPWQAACLGAYAHGLAGDLLAAAGGLEFGFTASELADRLPAAFGSFTKI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001940
EMBL· GenBank· DDBJ
ADH84953.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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