D6WW40 · D6WW40_TRICA
- ProteinEndonuclease III homolog
- GeneNTH1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids283 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 187 | Nucleophile; for N-glycosylase activity | ||||
Sequence: K | ||||||
Site | 206 | Important for catalytic activity | ||||
Sequence: D | ||||||
Binding site | 255 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 262 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 265 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 271 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity | |
Biological Process | base-excision repair, AP site formation | |
Biological Process | nucleotide-excision repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndonuclease III homolog
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Coleoptera > Polyphaga > Cucujiformia > Tenebrionidae > Tenebrionidae incertae sedis > Tribolium
Accessions
- Primary accessionD6WW40
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-58 | Disordered | ||||
Sequence: MEPSTSQVNKPDLSRFKMAPKRQLSHKSRDTKPDTQTKRGKRDHITIGSEIADKGPPD | ||||||
Compositional bias | 15-58 | Basic and acidic residues | ||||
Sequence: RFKMAPKRQLSHKSRDTKPDTQTKRGKRDHITIGSEIADKGPPD | ||||||
Domain | 105-253 | HhH-GPD | ||||
Sequence: LSSQTKDQVVFSAMQKLHKYGCNVDNILATSDEKLGELIYPVGFWKTKVKHIKKASEILKNEYNGDIPRTVEDLCKLPGVGPKMANLCMKTAWNEVTGIGVDTHVHRISNRIGWVKTKTPEETKKSLERWLPRDLWDEIGALLVGFGQQ |
Sequence similarities
Belongs to the Nth/MutY family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length283
- Mass (Da)31,999
- Last updated2016-05-11 v2
- ChecksumB0452AFFA01FBE5A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 15-58 | Basic and acidic residues | ||||
Sequence: RFKMAPKRQLSHKSRDTKPDTQTKRGKRDHITIGSEIADKGPPD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KQ971361 EMBL· GenBank· DDBJ | EFA08192.2 EMBL· GenBank· DDBJ | Genomic DNA |