D5X6A9 · D5X6A9_THIK1
- ProteinGlycerol-3-phosphate dehydrogenase [NAD(P)+]
- GenegpsA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids390 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reduction of the glycolytic intermediate dihydroxyacetone phosphate (DHAP) to sn-glycerol 3-phosphate (G3P), the key precursor for phospholipid synthesis.
Catalytic activity
- sn-glycerol 3-phosphate + NAD+ = dihydroxyacetone phosphate + NADH + H+
Pathway
Membrane lipid metabolism; glycerophospholipid metabolism.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23-28 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 27 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 47 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 48 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 121 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 121 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 121 | substrate | |||
Binding site | 150 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 152 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 154 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 154 | NADPH (UniProtKB | ChEBI) | |||
Active site | 218 | Proton acceptor | |||
Binding site | 218 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 271 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 281 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 282 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 282 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 282 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 282-283 | substrate | |||
Binding site | 283 | sn-glycerol 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 302 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 304 | NADPH (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | membrane | |
Molecular Function | glycerol-3-phosphate dehydrogenase [NAD(P)+] activity | |
Molecular Function | NAD binding | |
Biological Process | carbohydrate metabolic process | |
Biological Process | glycerol-3-phosphate biosynthetic process | |
Biological Process | glycerol-3-phosphate catabolic process | |
Biological Process | glycerophospholipid metabolic process | |
Biological Process | phospholipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlycerol-3-phosphate dehydrogenase [NAD(P)+]
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Thiomonas
Accessions
- Primary accessionD5X6A9
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 15-35 | Helical | |||
Keywords
- Cellular component
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 19-169 | Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal | |||
Domain | 207-343 | Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal | |||
Sequence similarities
Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length390
- Mass (Da)40,890
- Last updated2010-07-13 v1
- MD5 Checksum034F837BEABCB4DF210CC0549F9C0176
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002021 EMBL· GenBank· DDBJ | ADG32026.1 EMBL· GenBank· DDBJ | Genomic DNA |