D5X694 · D5X694_THIK1
- ProteindITP/XTP pyrophosphatase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids255 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.
Catalytic activity
- ITP + H2O = IMP + diphosphate + H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 15-20 | substrate | |||
Binding site | 47 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 76 | Proton acceptor | |||
Binding site | 76 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 77 | substrate | |||
Binding site | 164-167 | substrate | |||
Binding site | 186 | substrate | |||
Binding site | 191-192 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | dITP diphosphatase activity | |
Molecular Function | ITP diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside triphosphate phosphatase activity | |
Molecular Function | XTP diphosphatase activity | |
Biological Process | nucleotide metabolic process | |
Biological Process | purine nucleoside triphosphate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namedITP/XTP pyrophosphatase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Thiomonas
Accessions
- Primary accessionD5X694
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Sequence
- Sequence statusComplete
- Length255
- Mass (Da)26,826
- Last updated2010-07-13 v1
- MD5 Checksum255E647A03E1D9E069FEDECDC584590D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002021 EMBL· GenBank· DDBJ | ADG32011.1 EMBL· GenBank· DDBJ | Genomic DNA |