D5SME7 · D5SME7_PLAL2

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site77FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site97-100dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site100-102FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site108FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site108-112dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site180dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site196-198FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Active site207Involved in ionization of N3 of dUMP, leading to its activation
Binding site207dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionNADPH binding
Molecular Functionthymidylate synthase (FAD) activity
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-dependent thymidylate synthase
  • EC number
  • Short names
    FDTS
  • Alternative names
    • FAD-dependent thymidylate synthase
    • Thymidylate synthase ThyX
      (TS
      ; TSase
      )

Gene names

    • Name
      thyX
    • Ordered locus names
      Plim_0014

Organism names

Accessions

  • Primary accession
    D5SME7

Proteomes

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    313
  • Mass (Da)
    35,879
  • Last updated
    2010-07-13 v1
  • Checksum
    EFF629BDBD4C8233
MANSDQAPEEISRTALLEELRWKKFPVLDDGFVCLVDVMGDDSSIVQAARVSYGEGTKRVSDDRTLIRYLMRHRHSTPFEMAEVKLLVRVPMDCWRQWIRHRTANVNEYSTRYSVAIDSAQSTPIGEWRSQSTSNRQGSAGFLPNEIGAELTAAEERFQTAARELYESRLAQGVAREQARKDLPLCTYTEAYWKVDLHNLLGFLSLRMDSHAQYEIRTFAKTIGEQIIRPLFPVAWEAFLDYRFEGMFLSRLEVGMIQRLAERASAMKLVPPYSQADFLAVQDPTWAGLTRSRERDECLEKLVEMGIVQPAVE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001744
EMBL· GenBank· DDBJ
ADG65867.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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