D5SME7 · D5SME7_PLAL2
- ProteinFlavin-dependent thymidylate synthase
- GenethyX
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids313 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H+ + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP+
Cofactor
Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 77 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: T | ||||||
Binding site | 97-100 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: QWIR | ||||||
Binding site | 100-102 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: RHR | ||||||
Binding site | 108 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: E | ||||||
Binding site | 108-112 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: EYSTR | ||||||
Binding site | 180 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: R | ||||||
Binding site | 196-198 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: DLH | ||||||
Active site | 207 | Involved in ionization of N3 of dUMP, leading to its activation | ||||
Sequence: R | ||||||
Binding site | 207 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | NADPH binding | |
Molecular Function | thymidylate synthase (FAD) activity | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavin-dependent thymidylate synthase
- EC number
- Short namesFDTS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Planctomycetota > Planctomycetia > Planctomycetales > Planctomycetaceae > Planctopirus
Accessions
- Primary accessionD5SME7
Proteomes
Interaction
Structure
Sequence
- Sequence statusComplete
- Length313
- Mass (Da)35,879
- Last updated2010-07-13 v1
- ChecksumEFF629BDBD4C8233
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001744 EMBL· GenBank· DDBJ | ADG65867.1 EMBL· GenBank· DDBJ | Genomic DNA |