D4ZA31 · D4ZA31_BPAR1

Function

function

Involved in countering a host defense mechanism which, following viral infection, activates the host anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in the cleaved host tRNA.

Catalytic activity

  • ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.
    EC:6.5.1.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions that perform the catalytic activity via a two-metal mechanism. One of the catalytic Mg2+, which is coordinated by 5 water molecules, engages the lysine nucleophile and the ATP alpha phosphate while the Mg2+ orients the PPi leaving group.

Features

Showing features for binding site, active site, site.

137450100150200250300350
TypeIDPosition(s)Description
Binding site37ATP (UniProtKB | ChEBI)
Binding site54ATP (UniProtKB | ChEBI)
Binding site75ATP (UniProtKB | ChEBI)
Active site99N6-AMP-lysine intermediate
Binding site159ATP (UniProtKB | ChEBI)
Site159Essential for RNA ligase activity
Binding site240ATP (UniProtKB | ChEBI)
Binding site242ATP (UniProtKB | ChEBI)
Site246Essential for RNA ligase activity
Binding site272Mg2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Biological ProcessRNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA ligase 1
  • EC number
  • Alternative names
    • Rnl1

Gene names

    • Name
      rnlA
    • ORF names
      AR1_235

Organism names

Accessions

  • Primary accession
    D4ZA31

Proteomes

Structure

3D structure databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain52-246T4 RNA ligase 1-like N-terminal
Domain253-374T4 RNA ligase 1 C-terminal

Sequence similarities

Belongs to the Tequatrovirus RNA ligase 1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    43,451
  • Last updated
    2010-06-15 v1
  • Checksum
    0378DFCD25C0BC99
MQELFNNLMELCKDSQRKFFYSDDVSASGRTYRIFSYNYASYSDWLLPDALECRGIMFEMDGEKPVRIASRPMEKFFNLNENPFTMNIDLNDVDYILTKEDGSLVSTYLDGDEILFKSKGSIKSEQALMANGILMNINHHQLRDRLKELAEDGFTANFEFVAPTNRIVLAYQEMKIILLNIRENETGEYISYDDIYKDAALRPYLVERYEIDSPKWVEEAKNAENIEGYVAVMKDGSHFKIKSDWYVSLHSTKSSLDNPEKLFKTIIDGASDDLKAMYADDEYSYRKIEAFETTYLKYLDRALFLVLDCHNKHCGKDRKTYAMEAQGVAKGAGMDHLFGIIMSLYQGYDSQEKVMCEIEQNFLKNYKKFIPEGY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP011113
EMBL· GenBank· DDBJ
BAI83243.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp