D4VJH9 · D4VJH9_9BACE
- ProteinEndonuclease III
- Genenth
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids225 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 190 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 197 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 200 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 206 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA N-glycosylase activity | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, AP site formation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndonuclease III
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Bacteroidales > Bacteroidaceae > Bacteroides
Accessions
- Primary accessionD4VJH9
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length225
- Mass (Da)25,633
- Last updated2010-06-15 v1
- MD5 Checksum12E5DFF61F1F3C976135E957A6F0D61B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CBXG010000014 EMBL· GenBank· DDBJ | CDM03448.1 EMBL· GenBank· DDBJ | Genomic DNA |