D4VGI0 · D4VGI0_9BACE
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids268 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate
Pathway
Amino-sugar metabolism; N-acetylmuramate degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 78 | Proton donor | ||||
Sequence: E | ||||||
Active site | 109 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbohydrate derivative binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | ether hydrolase activity | |
Biological Process | amino sugar catabolic process | |
Biological Process | N-acetylmuramic acid catabolic process | |
Biological Process | peptidoglycan turnover |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Bacteroidales > Bacteroidaceae > Bacteroides
Accessions
- Primary accessionD4VGI0
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 50-213 | SIS | ||||
Sequence: IVPRMKQGGRIFYMGAGTSGRLGVLDASEIPPTFGMSPNWIIGLIAGGDTALRNPVEGAEDNENRGWEELVEHQINEKDTVIGIAASGTTPYVIGALREARRHGVLTGCITSNPDSPMAAEADVAIEMIVGPEYVTGSSRMKSGTGQKMILNMISTSVMIQLGR |
Sequence similarities
Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length268
- Mass (Da)29,338
- Last updated2010-06-15 v1
- ChecksumF22563DB7B53BEA2