D4S3H6 · D4S3H6_9FIRM
- ProteinAminodeoxyfutalosine synthase
- GenemqnE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids356 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
Catalytic activity
- 3-[(1-carboxyvinyl)-oxy]benzoate + S-adenosyl-L-methionine + H2O = 6-amino-6-deoxyfutalosine + hydrogencarbonate + L-methionine + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Quinol/quinone metabolism; menaquinone biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 60 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 64 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 67 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity | |
Molecular Function | aminodeoxyfutalosine synthase activity | |
Molecular Function | iron ion binding | |
Biological Process | menaquinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminodeoxyfutalosine synthase
- EC number
- Short namesAFL synthase ; Aminofutalosine synthase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Negativicutes > Selenomonadales > Selenomonadaceae > Selenomonas
Accessions
- Primary accessionD4S3H6
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 46-281 | Radical SAM core | |||
Sequence similarities
Belongs to the radical SAM superfamily. MqnE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)39,909
- Last updated2010-06-15 v1
- MD5 Checksum02BA07494370A2813A2DF541EC749DE0
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ACKT01000005 EMBL· GenBank· DDBJ | EFF67151.1 EMBL· GenBank· DDBJ | Genomic DNA |