D4GTS4 · JAMM1_HALVD

Function

function

Metalloprotease that displays desampylase (DSAMP) activity, cleaving ubiquitin-like small archaeal modifier proteins (SAMP1, SAMP2 and SAMP3) from protein conjugates (isopeptide- and linear-linked). Thus, likely regulates sampylation and the pools of 'free' SAMP available for protein modification. Functions as a specific and not a general protease since it is unable to hydrolyze a variety of unmodified proteins otherwise hydrolyzed by proteinase K.

Miscellaneous

Is optimally active at NaCl concentrations of 0.7-2 M, and displays little to no activity at low concentrations of salt (150 mM NaCl).

Catalytic activity

  • an N6-[small archaeal modifier protein]-[protein]-L-lysine + H2O = a [protein]-L-lysine + a [small archaeal modifier protein].
    EC:3.4.19.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Activity regulation

Inhibited by EDTA and N-ethylmaleimide (NEM) in vitro.

pH Dependence

Optimum pH is 7-10. Displays little to no activity at low pH (pH 6.5 and below).

Temperature Dependence

Optimum temperature is 40-50 degrees Celsius. Is active over a wide range of temperature (20-60 degrees Celsius). However, the enzyme is not active at 70 degrees Celsius.

Features

Showing features for active site, binding site, site.

TypeIDPosition(s)Description
Active site31Proton donor/acceptor
Binding site88Zn2+ (UniProtKB | ChEBI); catalytic
Binding site90Zn2+ (UniProtKB | ChEBI); catalytic
Site98Transition state stabilizer
Binding site101Zn2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular Functionmetalloexopeptidase activity
Molecular Functionzinc ion binding
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Desampylase
  • EC number
  • Alternative names
    • HvJAMM1

Gene names

    • ORF names
      C498_07813
    • Ordered locus names
      HVO_2505

Organism names

Accessions

  • Primary accession
    D4GTS4

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis31Loss of catalytic activity.
Mutagenesis88Loss of catalytic activity.
Mutagenesis90Loss of catalytic activity.
Mutagenesis94Retains desampylating activity.
Mutagenesis98Loss of catalytic activity.
Mutagenesis101Loss of catalytic activity.
Mutagenesis115Retains desampylating activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004289381-139Desampylase

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain6-139MPN
Motif88-101JAMM motif

Sequence similarities

Belongs to the peptidase M67B family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    139
  • Mass (Da)
    14,739
  • Last updated
    2010-05-18 v1
  • Checksum
    432D44455ADF0568
MTSSRLSLAADARDSILSHAREGAAGDPPAEVCGVLAGDSDARTVTAAHPVSNVAAEPRVAYELDPEETVSILEAIESAGDDAVGFYHSHPESDPVPSATDRERASWPGYVYLICSPDGRMTAHEWTGDEFRELSVAVE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001956
EMBL· GenBank· DDBJ
ADE04294.1
EMBL· GenBank· DDBJ
Genomic DNA
AOHU01000045
EMBL· GenBank· DDBJ
ELY32652.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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