D4GSE6 · KDGK1_HALVD
- Protein2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase
- GenekdgK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids320 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the degradation of glucose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG). Also catalyzes efficiently the phosphorylation of 2-keto-3-deoxygalactonate (KDGal) to 2-keto-3-deoxy-6-phosphogalactonate (KDPGal).
Catalytic activity
- 2-dehydro-3-deoxy-D-gluconate + ATP = 2-dehydro-3-deoxy-6-phospho-D-gluconate + ADP + H+This reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.54 mM | KDG | |||||
0.73 mM | KDGal | |||||
0.36 mM | ATP |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
78 μmol/min/mg | with KDG as substrate | ||||
33 μmol/min/mg | with KDGal as substrate |
Pathway
Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 34-38 | substrate | ||||
Sequence: GAESN | ||||||
Binding site | 92 | substrate | ||||
Sequence: Y | ||||||
Binding site | 107-109 | substrate | ||||
Sequence: YDR | ||||||
Binding site | 169-171 | ATP (UniProtKB | ChEBI) | ||||
Sequence: NYR | ||||||
Binding site | 171 | substrate | ||||
Sequence: R | ||||||
Binding site | 230-235 | ATP (UniProtKB | ChEBI) | ||||
Sequence: TRGEEG | ||||||
Binding site | 259-262 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GTGD | ||||||
Active site | 262 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 262 | substrate | ||||
Sequence: D | ||||||
Binding site | 298 | substrate | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 2-dehydro-3-deoxygalactonokinase activity | |
Molecular Function | 2-dehydro-3-deoxygluconokinase activity | |
Molecular Function | ATP binding | |
Biological Process | D-galacturonate catabolic process | |
Biological Process | D-glucuronate catabolic process | |
Biological Process | DNA damage response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase
- EC number
- Short names2-dehydro-3-deoxyglucono/galactono-kinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Haloferacaceae > Haloferax
Accessions
- Primary accessionD4GSE6
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Deletion of the gene abolishes growth on glucose, but does not affect growth on galactose. Double deletion mutant kdgK1/kdgK2 loses the ability to grow on galactose.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000449269 | 1-320 | 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase | |||
Sequence: MTALVTFGETMLRLSPPRGERLETARELEVQAGGAESNVAVAAARLGRDAAWFSKLPDSPLGRRIVSELRSHSVDTDGVVWTDDADARQGVYYLEHGASPRPTNVVYDRADAAVTTLETGEFDLDAVRDAEVCFTSGITPALSETLSETTADVLDEAQNAGTTTAFDLNYRTKLWSPDEAAEVYRDLLDSVDLLFAAERDAATVLGRDGDAESVARGLADDYDIETVVVTRGEEGSLAVSDGAVSEQGVYETETYDAIGTGDAFVGGFLAKHLDGGSVTESLEWASATASFKRTVEGDIAVVTPEDVERVVAEEGDGISR |
Proteomic databases
Expression
Induction
Constitutively expressed.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length320
- Mass (Da)34,083
- Last updated2010-05-18 v1
- Checksum98EB5BCFE982A17A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001956 EMBL· GenBank· DDBJ | ADE04815.1 EMBL· GenBank· DDBJ | Genomic DNA |