D4GSE6 · KDGK1_HALVD

Function

function

Involved in the degradation of glucose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG). Also catalyzes efficiently the phosphorylation of 2-keto-3-deoxygalactonate (KDGal) to 2-keto-3-deoxy-6-phosphogalactonate (KDPGal).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.54 mMKDG
0.73 mMKDGal
0.36 mMATP
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
78 μmol/min/mgwith KDG as substrate
33 μmol/min/mgwith KDGal as substrate

Pathway

Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 1/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site34-38substrate
Binding site92substrate
Binding site107-109substrate
Binding site169-171ATP (UniProtKB | ChEBI)
Binding site171substrate
Binding site230-235ATP (UniProtKB | ChEBI)
Binding site259-262ATP (UniProtKB | ChEBI)
Active site262Proton acceptor
Binding site262substrate
Binding site298substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function2-dehydro-3-deoxygalactonokinase activity
Molecular Function2-dehydro-3-deoxygluconokinase activity
Molecular FunctionATP binding
Biological ProcessD-galacturonate catabolic process
Biological ProcessD-glucuronate catabolic process
Biological ProcessDNA damage response

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase
  • EC number
  • Short names
    2-dehydro-3-deoxyglucono/galactono-kinase
  • Alternative names
    • 2-keto-3-deoxygluconate/2-keto-3-deoxygalactonate kinase
    • KDG kinase
    • KDGK-1

Gene names

    • Name
      kdgK1
    • Ordered locus names
      HVO_0549

Organism names

Accessions

  • Primary accession
    D4GSE6
  • Secondary accessions
    • L9UMS7

Proteomes

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Deletion of the gene abolishes growth on glucose, but does not affect growth on galactose. Double deletion mutant kdgK1/kdgK2 loses the ability to grow on galactose.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004492691-3202-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase

Proteomic databases

Expression

Induction

Constitutively expressed.

Interaction

Subunit

Homohexamer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    320
  • Mass (Da)
    34,083
  • Last updated
    2010-05-18 v1
  • Checksum
    98EB5BCFE982A17A
MTALVTFGETMLRLSPPRGERLETARELEVQAGGAESNVAVAAARLGRDAAWFSKLPDSPLGRRIVSELRSHSVDTDGVVWTDDADARQGVYYLEHGASPRPTNVVYDRADAAVTTLETGEFDLDAVRDAEVCFTSGITPALSETLSETTADVLDEAQNAGTTTAFDLNYRTKLWSPDEAAEVYRDLLDSVDLLFAAERDAATVLGRDGDAESVARGLADDYDIETVVVTRGEEGSLAVSDGAVSEQGVYETETYDAIGTGDAFVGGFLAKHLDGGSVTESLEWASATASFKRTVEGDIAVVTPEDVERVVAEEGDGISR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001956
EMBL· GenBank· DDBJ
ADE04815.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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