D4DX30 · D4DX30_SEROD
- Protein5-keto-4-deoxy-D-glucarate aldolase
- GenegarL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.
Catalytic activity
- 2-dehydro-3-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
- 5-dehydro-4-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 2/3.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 68 | Proton acceptor | ||||
Sequence: H | ||||||
Site | 93 | Transition state stabilizer | ||||
Sequence: R | ||||||
Site | 107 | Increases basicity of active site His | ||||
Sequence: D | ||||||
Binding site | 169 | substrate | ||||
Sequence: Q | ||||||
Binding site | 171 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 197 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 197 | substrate | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-dehydro-3-deoxyglucarate aldolase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | D-glucarate catabolic process | |
Biological Process | galactarate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-keto-4-deoxy-D-glucarate aldolase
- EC number
- Short namesKDGluc aldolase ; KDGlucA
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Serratia
Accessions
- Primary accessionD4DX30
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-264 | HpcH/HpaI aldolase/citrate lyase | ||||
Sequence: IGCWSALGNPISTEVLGLAGFDWLVLDGEHAPNDITTFVPQLMALKGSDSAAVVRPPCNEPVIIKRLLDIGFHNFLIPFVESEQQALQAVASTRYPPAGIRGVSVSHRSNGYGTVADYFATINDNVSVLVQIETQAGVDNLDAIAAVDGVDGIFVGPGDLSAALGYLGQPNHPEVQAVIRHIFDRAKTAGKPSGILAPAEADARRYLQWGAQFVAVGSDLGVLRNA |
Sequence similarities
Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)29,588
- Last updated2010-05-18 v1
- Checksum7A71B15B9A6E04BE
Keywords
- Technical term