D3ZSP7 · TTC3_RAT

  • Protein
    E3 ubiquitin-protein ligase TTC3
  • Gene
    Ttc3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

E3 ubiquitin-protein ligase which catalyzes the formation of 'Lys-48'-polyubiquitin chains (By similarity).
Mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus (By similarity).
Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation (By similarity).
Positively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation by mediating the ubiquitination and subsequent degradation of SMURF2 (By similarity).
Regulates neuronal differentiation by regulating actin remodeling and Golgi organization via a signaling cascade involving RHOA, CIT and ROCK (PubMed:24695496).
Inhibits cell proliferation (By similarity).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular ComponentGolgi apparatus
Cellular Componentnucleus
Cellular Componentvacuole
Molecular Functionmetal ion binding
Molecular Functionubiquitin-protein transferase activity
Biological Processnegative regulation of axon extension
Biological Processnegative regulation of neuron differentiation
Biological Processprotein K48-linked ubiquitination
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase TTC3
  • EC number
  • Alternative names
    • RING-type E3 ubiquitin transferase TTC3
    • TPR repeat protein D

Gene names

    • Name
      Ttc3

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    D3ZSP7

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Golgi apparatus
Note: Nuclear localization may be dependent on the proteolytic cleavage of full length protein in the cytoplasm (By similarity).
This cleavage may reveal an N-terminal nuclear localization signal, allowing N-terminal fragments to enter the nucleus (By similarity).

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00004486971-2000E3 ubiquitin-protein ligase TTC3
Modified residue397Phosphoserine
Modified residue1029Phosphoserine
Modified residue1080Phosphoserine

Post-translational modification

Phosphorylation on Ser-397 by Akt is required for ubiquitin ligase activity.
Proteolytically cleaved into differently sized N- and C-terminal fragments.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts (when phosphorylated on Ser-397) with AKT1, AKT2 and AKT3 (when phosphorylated) (By similarity).
Interacts with CIT (By similarity).
Interacts with POLG (By similarity).
Interacts with HSP70 (By similarity).
Interacts with SMURF2 (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, repeat, compositional bias, zinc finger.

Type
IDPosition(s)Description
Region20-249Interaction with POLG
Repeat250-283TPR 1
Repeat284-317TPR 2
Region442-478Disordered
Repeat556-592TPR 3
Repeat596-629TPR 4
Compositional bias804-826Basic and acidic residues
Region804-828Disordered
Region1041-1087Disordered
Region1233-1308Disordered
Compositional bias1266-1280Polar residues
Region1423-1448Disordered
Region1806-1839Disordered
Compositional bias1808-1839Polar residues
Compositional bias1894-1913Basic and acidic residues
Region1894-1947Disordered
Zinc finger1952-1991RING-type; atypical

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,000
  • Mass (Da)
    225,783
  • Last updated
    2015-07-22 v3
  • Checksum
    2716C704140F51AE
MPGHRGAPHLPPWVCGLCSMDDFAEGGLSLADDILLEDYPYEDDCICTPDFTTDDYVRVTQLYYEGVGMQYKDYAQSEKNLEYDICNIWCSKPLSILQDYCDAIKLYIFWPLLFQHQHSSIISRLHPCVEAIRSRAAEISLKKLQHLELMEDIVDLAKKVANDSFLIEGLLKIGYKIENKILAMEDALNWIKYTGDVTILPKLGSVDSGWPMLSIFFTEYKYHITRVVTENCNLLEEFRRHSCMQCVKQGELMKMRGNEEFAKEKFEIAVIYYTRAIEYRPENHLLYGNRALCFLRMGQFRNALSDGKRAIVLKNTWPKGHYRYCDALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHIKLQKQIEDLQGRTSNRNPIKAFYESRAYIPRNSSAPAFRTSLNFVETERGFRKTKYRMANGGGHQNQKVADEALKGDDCDCHPEFLPPPSQPPRHKGKQKSRNNESEKPSSNSQVTLQVDLKSILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAVSLLGIGRPEELSEAENQFKRIIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKAKTLICRLPGILTWPTSNVIIEESKPEKVKVMLEKFVEECKFPPVPDAVCCYQKCRGFSKIQIYLTDPDFKGFIRISCCQYCKVEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVVKEKAPSRPVLKQKCSSLEKLRLKEDKKLKRKIQKQEAKKLAQERMEEDLRESNPPKPEEPEETVESAQSCQFLDDRILQCIKQNADKIKSVVLNTSTLLKELLSWKVLSTEDYTTCFSSKNFVHEAVDYVIGHLIQEKNRVKTRIFLHVLSELKELDPKLAPWIQRLNSFGLDATGPFFTRYGASLKELDFNIVTFLWSEKYGHKLGSIEGKQLDYFCEPASVMEARCLIWLLEEHRDKFPALHSALDEFFDIMDSRCTVLRKQDSDEMPFGCIKVKNKGKKKKPKDSKPMLVGSGTASVTPSSETVTPEDHNRRNSDSAGPFAVPDHLRQDVEEFEALYDQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSDRMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVASRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFQPDVKSEAAAEDVTSIPGPADSSAPAAEDLKPQLDSDSSSGSASEDSRLEVVSPDLPTPLCEDASPSPTPAPEDAKPTYWTQSHLVTGFCTYLPFQGFGIAQSRPAYINMVPSLSQFTSIYTPLANISSEYPMPRSMPVVPSFVASERADGNAAAYFESHRLNTENASDDHTASETQILEGPLGMCVKSQSSTADARTALSEPEGNSRHSGSSDSLWEASLENVSGITDVPPAPSVAIQVSRSLIHQEVNTEPYAPFERQQGDLSQKEKECHLLREQLQVACEECEQMELRSSQETRDLEEKLQRHAEENKVSLPELQWALGKLDREIKKWHQEKKEIQERLKALKKKIKKVINTSDMSAQKNDGLDKECESQPDQSLGISSALTDEKAKVEESVRKGKELYEESQQRAVAAEVSVLENWKEREVCKLQGVATQAEACLKSLKLMNSDSAAYPDVECDILSWETFLSTVTEEIENTKCQFEEQIKAIKSGSRLSDLSSVQVSELLFPACSMIHPQLLSESSRHEDHGLVACVDSMTGAVLNDPYVGPDSGCSEEVPELSLGSPTHHPEGAQQLEVKKASQVSPSEQNPEADEKPSGQATRSSQSQKNPFNSIIEHLSVIFPCYASSELSGFIKKVRNKSKNSFSGLSIEEIVERVTEHIVEEQKKKKPNPGKDKKTSEAHPAASVSKSSPSPPLAAAGPSAKTKGQKKDDVPAPDGNSCQICHEIFKSKNMRVLKCGHKFHKGCFKQWLKGQSTCPTCGSSDLLSEE

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6AR04A0A8I6AR04_RATTtc31981
A0A8I6ABF8A0A8I6ABF8_RATTtc31963
A0A8I5Y675A0A8I5Y675_RATTtc31992
A0A8I5Y7R0A0A8I5Y7R0_RATTtc3311
A0A8L2QUD9A0A8L2QUD9_RATTtc32000
A0A8I6A4G2A0A8I6A4G2_RATTtc31626

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias804-826Basic and acidic residues
Compositional bias1266-1280Polar residues
Compositional bias1808-1839Polar residues
Compositional bias1894-1913Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AABR07033674
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AABR07033675
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AABR07033676
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

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