D3ZGB1 · NFAT5_RAT
- ProteinNuclear factor of activated T-cells 5
- GeneNfat5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1548 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes (PubMed:23233732).
Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (By similarity).
Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (By similarity).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 310-317 | |||||
Sequence: RARYLTEG |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNuclear factor of activated T-cells 5
- Short namesNF-AT5
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionD3ZGB1
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear distribution increases under hypertonic conditions (By similarity).
Recruited to sites of R-loop-associated DNA damage following poly-ADP-ribosylation by PARP1 (By similarity).
Recruited to sites of R-loop-associated DNA damage following poly-ADP-ribosylation by PARP1 (By similarity).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423618 | 1-1548 | Nuclear factor of activated T-cells 5 | |||
Sequence: MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPPRETSAASMSQTSGGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTSEKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNIFPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIFQVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMVMEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNGTQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTASSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTSNTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQGNNLTGSF | ||||||
Modified residue | 137 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 139 | N6-acetyllysine | ||||
Sequence: K | ||||||
Modified residue | 151 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 152 | Phosphothreonine; by CDK5 | ||||
Sequence: T | ||||||
Modified residue | 172 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 572 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate | ||||
Sequence: K | ||||||
Cross-link | 572 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate | ||||
Sequence: K | ||||||
Modified residue | 577 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 619 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K |
Post-translational modification
Phosphorylated. Phosphorylated at Thr-152 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.
Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Homodimer when bound to DNA, completely encircles its DNA target (By similarity).
Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (PubMed:23233732).
Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation (By similarity).
Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (PubMed:23233732).
Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 54-106 | Disordered | ||||
Sequence: QLPPPRETSAASMSQTSGGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSS | ||||||
Compositional bias | 60-74 | Polar residues | ||||
Sequence: ETSAASMSQTSGGEA | ||||||
Compositional bias | 86-106 | Polar residues | ||||
Sequence: ASSAPSSSMGGACSSFTTSSS | ||||||
Compositional bias | 141-155 | Polar residues | ||||
Sequence: LTGNTVQQHPSTPKR | ||||||
Region | 141-180 | Disordered | ||||
Sequence: LTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEG | ||||||
Compositional bias | 192-212 | Polar residues | ||||
Sequence: WMEDSPSNFSNMSTSSYNDNT | ||||||
Region | 192-237 | Disordered | ||||
Sequence: WMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEES | ||||||
Compositional bias | 258-278 | Polar residues | ||||
Sequence: TTDNKGNSKAGNGTLDSQKGT | ||||||
Region | 258-282 | Disordered | ||||
Sequence: TTDNKGNSKAGNGTLDSQKGTGVKK | ||||||
Domain | 281-460 | RHD | ||||
Sequence: KKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAG | ||||||
Region | 659-682 | Disordered | ||||
Sequence: GNASFSSPSSSHLSPENENQQQLQ | ||||||
Region | 750-777 | Disordered | ||||
Sequence: TEAPQQQQSPLQEQAQIPSNIFPSPNSV | ||||||
Region | 851-892 | Disordered | ||||
Sequence: PGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQ | ||||||
Compositional bias | 873-892 | Polar residues | ||||
Sequence: ADSVHQQTENTLSSQQQQQQ | ||||||
Region | 970-1010 | Disordered | ||||
Sequence: SPPAVSGNETSTTTTPQVATPGSTMFQPPNSGDGEETGAQA | ||||||
Region | 1097-1127 | Disordered | ||||
Sequence: LSQETQGPMFHSANPIVHSQTSTASSEQLQP | ||||||
Region | 1257-1388 | Disordered | ||||
Sequence: MQSNSPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQ |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,548
- Mass (Da)167,207
- Last updated2010-04-20 v1
- ChecksumCE3F4A41532770EF
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2K1M9 | A0A0G2K1M9_RAT | Nfat5 | 217 | ||
A0A8I6ANN3 | A0A8I6ANN3_RAT | Nfat5 | 1476 | ||
A0A8I5ZWM0 | A0A8I5ZWM0_RAT | Nfat5 | 1530 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 60-74 | Polar residues | ||||
Sequence: ETSAASMSQTSGGEA | ||||||
Compositional bias | 86-106 | Polar residues | ||||
Sequence: ASSAPSSSMGGACSSFTTSSS | ||||||
Compositional bias | 141-155 | Polar residues | ||||
Sequence: LTGNTVQQHPSTPKR | ||||||
Compositional bias | 192-212 | Polar residues | ||||
Sequence: WMEDSPSNFSNMSTSSYNDNT | ||||||
Compositional bias | 258-278 | Polar residues | ||||
Sequence: TTDNKGNSKAGNGTLDSQKGT | ||||||
Compositional bias | 873-892 | Polar residues | ||||
Sequence: ADSVHQQTENTLSSQQQQQQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AABR06098525 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AABR06098526 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AABR06098527 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH473972 EMBL· GenBank· DDBJ | EDL92476.1 EMBL· GenBank· DDBJ | Genomic DNA |