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D3V8R3 · D3V8R3_XENNA

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site94-107NAD+ (UniProtKB | ChEBI)
Binding site115substrate
Binding site125substrate
Binding site154substrate
Site161Important for catalysis
Site211Important for catalysis
Binding site243Mg2+ (UniProtKB | ChEBI)
Binding site243substrate
Binding site267Mg2+ (UniProtKB | ChEBI)
Binding site271Mg2+ (UniProtKB | ChEBI)
Binding site301-313NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • Ordered locus names
      XNC1_1040

Organism names

Accessions

  • Primary accession
    D3V8R3

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain22-371Isopropylmalate dehydrogenase-like

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    389
  • Mass (Da)
    43,187
  • Last updated
    2010-04-20 v1
  • MD5 Checksum
    D9E2763CC4CAD7AE6B44C194CDBDEDC0
MSSSQSISNNTIANKQKANHFHIAVLPGDGIGPEVMKQAYKILDAIRHRFNIRITTSEYDIGGIAIDRHGSPLPQETVIGCEKADAILFGSVGGPKWEHLPPSQQPERGALLPLRKHFKLFSNLRPARLYRGLESFCPLRHDIATKGFDILCVRELTGGIYFGQPKGREGKGKYERAFDTEIYHRFEIERIAHIAFQSARKRRHKVTSIDKANVLQSSVLWREIVSEIAKDYPDVEINHMYIDNATMQLIKNPAQFDVLLCSNIFGDILSDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKNIANPIAQILSVALLLRYSFECNDAANAIEQAINQALEQGYRTADLTSNDNTISTDEMGDIIARYITEEYRKTISLSSLT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN667742
EMBL· GenBank· DDBJ
CBJ89111.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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