D3NWP5 · D3NWP5_AZOS1
- ProteinUrease subunit alpha
- GeneureC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids569 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- urea + 2 H2O + H+ = hydrogencarbonate + 2 NH4+
Cofactor
Note: Binds 2 nickel ions per subunit.
Pathway
Nitrogen metabolism; urea degradation; CO2 and NH3 from urea (urease route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 136 | Ni2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 138 | Ni2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 219 | Ni2+ 1 (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 219 | Ni2+ 2 (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 221 | substrate | |||
Binding site | 248 | Ni2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 274 | Ni2+ 2 (UniProtKB | ChEBI) | |||
Active site | 322 | Proton donor | |||
Binding site | 362 | Ni2+ 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | nickel cation binding | |
Molecular Function | urease activity | |
Biological Process | urea catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameUrease subunit alpha
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Azospirillaceae > Azospirillum
Accessions
- Primary accessionD3NWP5
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 219 | N6-carboxylysine | |||
Post-translational modification
Carbamylation allows a single lysine to coordinate two nickel ions.
Carboxylation allows a single lysine to coordinate two nickel ions.
Interaction
Subunit
Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 131-569 | Urease | |||
Sequence similarities
Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length569
- Mass (Da)61,049
- Last updated2010-04-20 v1
- MD5 ChecksumB3856E3D3CF4B64078EDCD029917F8D3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP010946 EMBL· GenBank· DDBJ | BAI72824.1 EMBL· GenBank· DDBJ | Genomic DNA |