D3E3M2 · ARFB_METRM
- Protein2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase
- GenearfB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids234 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the hydrolysis of the formamide of 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-monophosphate (FAPy) to form 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (APy).
Catalytic activity
- 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + H2O = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate + H+
Cofactor
Protein has several cofactor binding sites:
Note: Requires one Fe2+ ion for activity.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )
Note: Requires an additional second metal ion that could be Fe2+ or Zn2+.
Pathway
Cofactor biosynthesis; coenzyme F420 biosynthesis.
Cofactor biosynthesis; riboflavin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 29 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 31 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 40 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 40 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 109 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-amino-5-formylamino-6-(5-phosphoribosylamino)pyrimidin-4(3H)-one formate-lyase activity | |
Molecular Function | ferrous iron binding | |
Biological Process | F420-0 metabolic process | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase
- EC number
- Short namesFAPy deformylase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanobrevibacter
Accessions
- Primary accessionD3E3M2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000406918 | 1-234 | 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase | |||
Sequence: MVELRVNAGNVKNPNVHKIGIIALGSHLENHGPALPIDTDAKIASHIAFQASLESGAKFLGVIYPAHELKEINHGIHVSLEDLTDEIVKVLKSAKKFLRISSVIIVNGHGGNLPIVTTLYDIEERTGLLITLNSKIIESEGPHGGSGELSMAKALGIIDESQVENQTNLEEYGEVGLYMFGEARRNDPNIEEGALDVEENGVYVDDVYGQELLKLAINSVLLDVEKQLDSHYGY |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length234
- Mass (Da)25,457
- Last updated2010-03-23 v1
- Checksum86D2559AF142AED3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001719 EMBL· GenBank· DDBJ | ADC47133.1 EMBL· GenBank· DDBJ | Genomic DNA |