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D3E0J6 · D3E0J6_METRM

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

Type
IDPosition(s)Description
Site53Interaction with DNA
Site165Interaction with DNA
Site169Interaction with DNA
Active site313O-(5'-phospho-DNA)-tyrosine intermediate
Site315Interaction with DNA
Site498Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • Ordered locus names
      mru_0390

Organism names

Accessions

  • Primary accession
    D3E0J6

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain2-144Toprim
Region195-200Interaction with DNA
Region720-741Disordered

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    741
  • Mass (Da)
    83,580
  • Last updated
    2010-03-23 v1
  • MD5 Checksum
    61D9280B1D1C8E490EC4BF18ECE1C51E
MHEVIISEKPKSAEKIAKALSPNAQKKKYNRKINYWEFEENGKKTTVLSAVGHLYSLTSASSRDRLGFDLTWVPAYEVEKQKYTRDYINAIKKLSKGADKFVHACDYDVEGTLIGYNALKYACGNNAEAKASRMKFSTLTKKDIVEAYNTMRDIDIRQVDHGIARHMLDYYYGMNISSALMKAVRASSSRFISLSAGRVQTPTLSILVDREKEIQSFVPEPYWMIKAKSDYDIIADHVEGKIFDEERAKKIYADCQGADADVTSVKVTESIRKPPIPFNLGGLQSEAHTVFGFSPKRTQVAAQNLYVGGYTSYPRTSSQKLPESLDFKNIFAGLAKDPEFKKHIFDLPAKLKPNEGKKTDAAHPAIHPTGVLPSNLSADEAKIYSLIVYRFISVFSENSKLETMKVNLKVADEKFTFSRKRVSYEGWLKHYPFKKQENDAFPPINKGDQLKIHKILVEEKETKPPARYNEASLIKELEKRELGTKATRADIVAKLYDRKYIEGKKIEVKPLGINIIDTLNEYCKDLTSEELTREFERELEGIDTDKYTKEQILENGEKEVKTILAEINQNKKQIGEKLYGAYQETNVVGECSCGGKLIKRYSPRTKNTFVGCSNFPKCKVTYSLPKGANMLKKTCPTCGLPMISIKKASGKGRDHVCLDSNCGKDVSRRQAPEVVGKCPECGKDLLKRSGRYGEFVGCSGFPRCRFTCSVDELNNLGKDKNKEDVKENKGVSKKIVRSEAK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001719
EMBL· GenBank· DDBJ
ADC46242.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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