D2Z030 · DCSG_STRLA

  • Protein
    Cycloserine biosynthesis protein DcsG
  • Gene
    dcsG
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the synthesis of D-cycloserine from O-ureido-D-serine (D-OUS). It reacts with D-OUS, D-homocysteine and beta-aminooxy-D-alanine.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ions per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.3 mMO-ureido-D-serine (D-OUS)
10 mMD-OUS in the presence of 5 mM ATP
19 mMD-homocysteine
73 μMATP, in the presence of 50 mM D-OUS
kcat is 2200 sec-1 with D-OUS as substrate. kcat is 0.86 sec-1 with D-homocysteine as substrate.

pH Dependence

Optimum pH is 8.0.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site92ATP (UniProtKB | ChEBI)
Binding site137ATP (UniProtKB | ChEBI)
Binding site144ATP (UniProtKB | ChEBI)
Binding site175ATP (UniProtKB | ChEBI)
Binding site176ATP (UniProtKB | ChEBI)
Binding site178ATP (UniProtKB | ChEBI)
Active site220
Active site254
Binding site269Mg2+ 1 (UniProtKB | ChEBI)
Binding site269Mg2+ 2 (UniProtKB | ChEBI)
Active site271
Binding site271Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncyclo-ligase activity
Molecular Functionmetal ion binding
Biological Processantibiotic biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cycloserine biosynthesis protein DcsG
  • EC number

Gene names

    • Name
      dcsG

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 11924 / 8197-20
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    D2Z030

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene do not produce cycloserine (DCS).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis57Loss of enzyme activity.
Mutagenesis142About 80% activity on D-OUS, 40% on D-homocysteine and beta-aminooxy-D-alanine.
Mutagenesis143About 15% activity on D-OUS, 5% on D-homocysteine and 30% on beta-aminooxy-D-alanine.
Mutagenesis202Nearly complete loss of activity on all substrates.
Mutagenesis215About 5% activity on D-OUS and beta-aminooxy-D-alanine, nearly complete loss of activity on D-homocysteine.
Mutagenesis220Nearly complete loss of activity on D-OUS, about 5% activity on D-homocysteine and beta-aminooxy-D-alanine.
Mutagenesis254Nearly complete loss of activity on all substrates.
Mutagenesis271Nearly complete loss of activity on all substrates.
Mutagenesis276About 5% activity on D-OUS, 50% on D-homocysteine and 20% beta-aminooxy-D-alanine.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004240591-299Cycloserine biosynthesis protein DcsG

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain95-298ATP-grasp

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    299
  • Mass (Da)
    32,786
  • Last updated
    2010-03-23 v1
  • Checksum
    8E4784DF256B9B23
MGILALVTDAVSLPIDYDMPPLLEACRTVGITAEVCDWEDGTVDWSRFEAVVFRSPWTWAERQAEFLAFCERVSHVTRLITPMPLVRWALDKRYLADLAAHGVPVIPTTVVAPGSDALAAVRDFLAARPEAREFVVKPTDGCYSKDVQRYQRSLAEPASRHVARLLANGSHVILQPYVESVDRHGETDLTFFDGVYSHAIHKGAMLMPDGTVHVPTLDFRQARDADEDQRAVAAAALAASVAHLGLDLPLVCGRVDLVRGADGSPMVLEMELCEPSLNLTFSEDGALRFAQALAERLKP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB516431
EMBL· GenBank· DDBJ
BAI70381.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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