D2Y1X8 · H1A03_CYRHA
- ProteinMu-theraphotoxin-Hhn2b 3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Weakly blocks the rat SCN2A/SCN1B (Nav1.2/beta-1) sodium channel (IC50=68 uM) and the insect sodium channel para/tipE (IC50=4.3 uM), without altering the activation or inactivation kinetics (depressant toxin).
Miscellaneous
Negative results: has no effect on mammalian SCN3A/SCN1B (Nav1.3/beta-1), SCN4A (Nav1.4), SCN5A/SCN1B (Nav1.5/beta-1), and SCN9A/SCN1B (Nav1.7/beta-1) (PubMed:14675784, PubMed:26429937).
Has also no effect on sodium subtypes in rat DRG neurons containing Nav1.1/SCN1A, Nav1.6/SCN8A, Nav1.7/SCN9A, Nav1.8/SCN10A and Nav1.9/SCN11A (PubMed:14675784).
Has also no effect on sodium subtypes in rat DRG neurons containing Nav1.1/SCN1A, Nav1.6/SCN8A, Nav1.7/SCN9A, Nav1.8/SCN10A and Nav1.9/SCN11A (PubMed:14675784).
Klint et al., 2015 worked with a recombinant peptide N-terminally extended by an Ala residue (AECKGFGKSCVPGKNECCSGYACNSRDKWCKVLL).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | ion channel inhibitor activity | |
Molecular Function | sodium channel regulator activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameMu-theraphotoxin-Hhn2b 3
- Short namesMu-TRTX-Hhn2b
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Mygalomorphae > Theraphosidae > Haplopelma
Accessions
- Primary accessionD2Y1X8
- Secondary accessions
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 51 | No gain of activity against hNav1.7/SCN9A. | ||||
Sequence: K → L | ||||||
Mutagenesis | 54 | Low gain of inhibition activity against hNav1.7/SCN9A (IC50=2.7 uM), and gain of binding to anionic POPG liposome. Important gain of inhibition activity (IC50=440 nM), and gain of binding to anionic POPG liposome; when associated with S-71. | ||||
Sequence: G → W | ||||||
Mutagenesis | 71 | Low gain of inhibition activity against hNav1.7/SCN9A (IC50=4.0 uM) and no change in binding to anionic POPG liposome. Important gain of inhibition activity (IC50=440 nM), and gain of binding to anionic POPG liposome; when associated with W-54. | ||||
Sequence: N → S | ||||||
Mutagenesis | 79 | No gain of activity against hNav1.7/SCN9A. | ||||
Sequence: V → W |
PTM/Processing
Features
Showing features for signal, propeptide, peptide, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MKASMFLALAGLVLLFVVCYA | ||||||
Propeptide | PRO_0000400498 | 22-48 | ||||
Sequence: SESEEKEFPRELISKIFAVDDFKGEVR | ||||||
Peptide | PRO_0000400499 | 49-81 | Mu-theraphotoxin-Hhn2b 3 | |||
Sequence: ECKGFGKSCVPGKNECCSGYACNSRDKWCKVLL | ||||||
Disulfide bond | 50↔65 | |||||
Sequence: CKGFGKSCVPGKNECC | ||||||
Disulfide bond | 57↔70 | |||||
Sequence: CVPGKNECCSGYAC | ||||||
Disulfide bond | 64↔77 | |||||
Sequence: CCSGYACNSRDKWC | ||||||
Modified residue | 81 | Leucine amide | ||||
Sequence: L |
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Domain
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Sequence similarities
Belongs to the neurotoxin 10 (Hwtx-1) family. 14 (Hntx-1) subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length83
- Mass (Da)9,210
- Last updated2010-03-02 v1
- ChecksumA72E5B88A8ACCDF2
Mass Spectrometry
Keywords
- Technical term