D2TU05 · D2TU05_CITRI

Function

function

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.
heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Features

Showing features for site, binding site, active site.

Type
IDPosition(s)Description
Site29Involved in heme-bound ligand stabilization and O-O bond activation
Site84Influences the redox potential of the prosthetic heme and FAD groups
Binding site85Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); proximal binding residue
Active site95Charge relay system
Active site135Charge relay system
Binding site188FAD (UniProtKB | ChEBI)
Binding site204-207FAD (UniProtKB | ChEBI)
Binding site268-273NADP+ (UniProtKB | ChEBI)
Site388Influences the redox potential of the prosthetic heme and FAD groups
Binding site389-392FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionnitric oxide dioxygenase NAD(P)H activity
Molecular Functionoxygen binding
Molecular Functionoxygen carrier activity
Biological Processcellular response to nitrosative stress
Biological Processnitric oxide catabolic process
Biological Processresponse to toxic substance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Flavohemoprotein
  • Alternative names
    • Flavohemoglobin
    • Hemoglobin-like protein
    • Nitric oxide dioxygenase
      (NO oxygenase
      ; NOD
      ) (EC:1.14.12.17
      ) . EC:1.14.12.17 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      hmpA
    • Synonyms
      hmp
    • Ordered locus names
      ROD_24941

Organism names

Accessions

  • Primary accession
    D2TU05

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-134Globin
Region147-396Reductase
Domain150-255FAD-binding FR-type

Domain

Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.

Sequence similarities

Belongs to the globin family. Two-domain flavohemoproteins subfamily.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    396
  • Mass (Da)
    43,543
  • Last updated
    2010-03-02 v1
  • Checksum
    12C1162D69075F1D
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNLENLPALLPAVEKIAQKHTSFQIQPEQYNIVGTHLLATLDEMFSPGQEVLDAWGKAYGVLANVFINREAQIYSENASKTGGWEGTRPFRIVAKTPRSALITSFEFEPVDGGAVADYQPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHNQANVGDIVHLAAPAGDFFMSVATDTPVSLISAGVGQTPMLAMLDTLAKAKHTAQVNWFHAAENGDVHAFADEVNELGSTLANFTAHTWYRVPTEADRAKAVFDSEGLMDLTTMKAAIANPAMQFYLCGPVGFMQFAAKQLVELGVNSENIHYECFGPHKVL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN543502
EMBL· GenBank· DDBJ
CBG89237.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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