D2QBD6 · D2QBD6_SPILD

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

111481002003004005006007008009001,0001,100
TypeIDPosition(s)Description
Binding site697ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • Ordered locus names
      Slin_0044

Organism names

Accessions

  • Primary accession
    D2QBD6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain23-731Aminoacyl-tRNA synthetase class Ia
Motif53-63'HIGH' region
Motif694-698'KMSKS' region
Domain781-933Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,148
  • Mass (Da)
    130,229
  • Last updated
    2010-03-02 v1
  • Checksum
    3D6AF9F203BFA62C
MILSDVKYQQYESLDYAQIAAEVLTYWNQNQVFEKSVSIREGKPSFTFYEGPPSANGTPGIHHVMARSIKDIFCRYKTLKGFQVERKGGWDTHGLPIELQVEKELGITKDDIGKPESEGGISVEEYNRKCRETVMRFTDQWNSLTEKMGYWVDLENPYITYKNEYIESVWSLLKKLYDQDLLYKGYTIQPYSPKAGTGLSSHELNMPGTYKDVRDTTIVAQFKVKPTGKSGFLFFDSADADVYILAWTTTPWTLPANSALTVGANIDYVLVKTFNPYTHIPVHVVLAKNLVGRWLNEKGQVDAVNSPAFDAYLPNDKVIPWTIVSEFKGQHLEGIEYEQLMPYVQPDKPAFRVILGDFVTTEDGTGVVHTSPTFGADDFRVAQAAGIPAIMVKDETGKEVPVVDKHGQFVKEIGEFGGRYVKEEYYSDEEREDPEFKPTDVLIAIKLKEENKAFRVEKYEHPYPHCWRTDKPILYYPLDSWFIRTTAKKDRLVELNKTINWQPESTGTGRFGNWLENLVDWNLSRSRFWGTPLPIWRSESGEEVCVGSIEELTELASQAVSSGKLTEEQQAKNKSFIDAVVAHKLNPEIEFDLHRPYVDEIYFVSESGQVMQRESDLIDVWFDSGAMPYAQWHYPFENQDVFNKAFPADFISEGVDQTRGWFFTLHAIAGMLFDSVAFKNVVSTGLVLDKNGNKMSKRLGNAVNPFETLDKYGADATRWYMITNAEPWDNLKFNTDGIGEVQRKLFGTLSNTYNFFALYANLDQFTFAGRTVPVAERPELDRWILSKLQSLILAVEEQFDSYNPTKAGRLVQDFVNDQLSNWYVRLSRRRFWTGAAPDGELTTDKRAAYETLHECLSTVSQLMSPIAPFYADWLYRNLNEGAVSVHLTDFPVGDHTLIDAELERSMELAQQVSSLVHSLRKGHKLKVRQPLSRVLVPVLNTDTRRQIEHVTPIIMSEVNVKAVEFVDDASGILKKKVKPNFKALGPKFGKQMKDVAAGITAMSAEELKGLEQTGEFVIPSLNQSITLADVEILTEDIPGWLVASEDGITVALDVTLSDELRGEGIARDFVNRIQNLRKDRDFSVTDKIRITLENNHDLLAKAVESNSAYIRQEVQAVSLDLVNDLNGSPAVAEIEMDEFQLRVLVDKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001769
EMBL· GenBank· DDBJ
ADB36118.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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