D2JBE4 · D2JBE4_ENTFC
- ProteinD-alanine--D-alanine ligase
- GenevanA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids343 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Cell wall formation.
Catalytic activity
- 2 D-alanine + ATP = D-alanyl-D-alanine + ADP + phosphate + H+
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 16 | ||||
Binding site | 133 | ATP (UniProtKB | ChEBI) | |||
Binding site | 169-171 | ATP (UniProtKB | ChEBI) | |||
Active site | 177 | ||||
Binding site | 177-178 | ATP (UniProtKB | ChEBI) | |||
Binding site | 207-214 | ATP (UniProtKB | ChEBI) | |||
Binding site | 292 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 304-305 | ATP (UniProtKB | ChEBI) | |||
Binding site | 305 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 305 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 307 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Active site | 316 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | D-alanine-D-alanine ligase activity | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-alanine--D-alanine ligase
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid p5753cA
- Plasmid p87_1_57kb
- Plasmid pF856
- Plasmid pS177
Organism names
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus
Accessions
- Primary accessionD2JBE4
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusComplete
- Length343
- Mass (Da)37,415
- Last updated2010-02-09 v1
- Checksum5AA4993B24F0056E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GQ900435 EMBL· GenBank· DDBJ | ADA62243.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HQ115078 EMBL· GenBank· DDBJ | ADV60062.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JQ663598 EMBL· GenBank· DDBJ | AFV27319.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KR349520 EMBL· GenBank· DDBJ | AKL90235.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WEFP01000006 EMBL· GenBank· DDBJ | KAB7572422.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WEGG01000006 EMBL· GenBank· DDBJ | KAB7614050.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MVGJ01000665 EMBL· GenBank· DDBJ | OOL76090.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP066461 EMBL· GenBank· DDBJ | QQF34307.1 EMBL· GenBank· DDBJ | Genomic DNA |