D2GXM8 · CBPC5_AILME
- ProteinCytosolic carboxypeptidase-like protein 5
- GeneAGBL5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids884 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Cleaves alpha- and gamma-linked polyglutamate tubulin side-chain, as well as the branching point glutamate. Also catalyzes the removal of alpha-linked glutamate residues from the carboxy-terminus of alpha-tubulin. Mediates deglutamylation of nucleotidyltransferase CGAS, leading to CGAS antiviral defense response activation.
Catalytic activity
- gamma-L-glutamyl-L-glutamyl-[protein] + H2O = L-glutamate + L-glutamyl-[protein]This reaction proceeds in the forward direction.
- (L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamateThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | intercellular bridge | |
Cellular Component | midbody | |
Cellular Component | mitotic spindle | |
Cellular Component | nucleus | |
Molecular Function | metallocarboxypeptidase activity | |
Molecular Function | tubulin binding | |
Molecular Function | zinc ion binding | |
Biological Process | C-terminal protein deglutamylation | |
Biological Process | defense response to virus | |
Biological Process | protein branching point deglutamylation | |
Biological Process | protein deglutamylation | |
Biological Process | protein side chain deglutamylation | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic carboxypeptidase-like protein 5
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Ursidae > Ailuropoda
Accessions
- Primary accessionD2GXM8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Mainly cytoplasmic. Slight accumulation in the nucleus is observed. Colocalizes with alpha-tubulin in the mitotic spindle and with midbody microtubules in the intercellular bridges formed during cytokinesis.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000403761 | 1-884 | Cytosolic carboxypeptidase-like protein 5 | |||
Sequence: MELRCGGLLFSSRFDSGNLAHVEKVDSVSGDGEGGAAGASAPFSSIASSPDYEFNVWTRPDCAETEFENGNRSWFYFSVRGGTPGKLIKINIMNMNKQSKLYSQGMAPFVRTLPTRPRWERIRDRPTFEMTETQFVLSFVHRFVEGRGATTFFAFCYPFSYSDCQDLLNQLDQRFLENHPTHSSPLDTIYYHREILCYSLDGLRVDLLTITSCHGLREDREPRLQQLFPDTGTPRPFCFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHYRTDSRGVNLNRQYLKPDAALHPAIYGAKAVLLYHHVHSRLHPQSPSEHQHSPCLPPDAPLSDLEKANHLRNEAHLGHTSDGDSPEDWTQTRPAEQKASGVWLMPQQCADAEQPAPDTIPPKESGVAYYVDLHGHASKRGCFMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSEHSSLTNLRAWMLKHVRSSRGLSSTVSGAVNKKRGSRTPPRSNSGLPVSCSENPLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPTGLPGLGSSTQKVSHRVLGPVREPRSQDRRRRQQPLTHRPTSSSLAPSPNPTSSSPASSHSTGPCLLPSAFSVSGSSCSLLSSGDKPEAVMVIGKGLLGPRIPCIRTRLQVRPRLGQGSPPTCRGMSGSSGPTSPIPRTRESSEPEPGPHSAPGLPQAGPPRPRSAPAFSPISCSLSDSQSRICYSGGPLGQPEVCFGPKSPPLTVSPRV | ||||||
Modified residue | 839 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 157-570 | Peptidase M14 | ||||
Sequence: YPFSYSDCQDLLNQLDQRFLENHPTHSSPLDTIYYHREILCYSLDGLRVDLLTITSCHGLREDREPRLQQLFPDTGTPRPFCFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHYRTDSRGVNLNRQYLKPDAALHPAIYGAKAVLLYHHVHSRLHPQSPSEHQHSPCLPPDAPLSDLEKANHLRNEAHLGHTSDGDSPEDWTQTRPAEQKASGVWLMPQQCADAEQPAPDTIPPKESGVAYYVDLHGHASKRGCFMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMA | ||||||
Region | 343-364 | Disordered | ||||
Sequence: HPQSPSEHQHSPCLPPDAPLSD | ||||||
Region | 376-401 | Disordered | ||||
Sequence: HLGHTSDGDSPEDWTQTRPAEQKASG | ||||||
Compositional bias | 603-670 | Polar residues | ||||
Sequence: LSSTVSGAVNKKRGSRTPPRSNSGLPVSCSENPLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFH | ||||||
Region | 603-737 | Disordered | ||||
Sequence: LSSTVSGAVNKKRGSRTPPRSNSGLPVSCSENPLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPTGLPGLGSSTQKVSHRVLGPVREPRSQDRRRRQQPLTHRPTSSSLAPSPNPTSSSPASSHSTG | ||||||
Compositional bias | 692-709 | Basic and acidic residues | ||||
Sequence: LGPVREPRSQDRRRRQQP | ||||||
Compositional bias | 711-737 | Polar residues | ||||
Sequence: THRPTSSSLAPSPNPTSSSPASSHSTG | ||||||
Region | 784-859 | Disordered | ||||
Sequence: QVRPRLGQGSPPTCRGMSGSSGPTSPIPRTRESSEPEPGPHSAPGLPQAGPPRPRSAPAFSPISCSLSDSQSRICY | ||||||
Compositional bias | 796-814 | Polar residues | ||||
Sequence: TCRGMSGSSGPTSPIPRTR | ||||||
Compositional bias | 823-841 | Pro residues | ||||
Sequence: PHSAPGLPQAGPPRPRSAP | ||||||
Compositional bias | 844-859 | Polar residues | ||||
Sequence: SPISCSLSDSQSRICY |
Sequence similarities
Belongs to the peptidase M14 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length884
- Mass (Da)96,914
- Last updated2010-02-09 v1
- Checksum0217A5C6E56BB2B2
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 603-670 | Polar residues | ||||
Sequence: LSSTVSGAVNKKRGSRTPPRSNSGLPVSCSENPLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFH | ||||||
Compositional bias | 692-709 | Basic and acidic residues | ||||
Sequence: LGPVREPRSQDRRRRQQP | ||||||
Compositional bias | 711-737 | Polar residues | ||||
Sequence: THRPTSSSLAPSPNPTSSSPASSHSTG | ||||||
Compositional bias | 796-814 | Polar residues | ||||
Sequence: TCRGMSGSSGPTSPIPRTR | ||||||
Compositional bias | 823-841 | Pro residues | ||||
Sequence: PHSAPGLPQAGPPRPRSAP | ||||||
Compositional bias | 844-859 | Polar residues | ||||
Sequence: SPISCSLSDSQSRICY |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GL192369 EMBL· GenBank· DDBJ | EFB20547.1 EMBL· GenBank· DDBJ | Genomic DNA |