D1YV03 · D1YV03_METPS

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site14diphosphate (UniProtKB | ChEBI)
Binding site106Mg2+ (UniProtKB | ChEBI); catalytic
Site107Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site127Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site128-130substrate; ligand shared between dimeric partners; in other chain
Active site130Proton acceptor
Binding site165substrate; ligand shared between dimeric partners
Binding site172-174substrate; ligand shared between dimeric partners; in other chain
Binding site225substrate; ligand shared between dimeric partners; in other chain
Binding site269substrate; ligand shared between dimeric partners
Binding site275-278substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      pfp
    • Ordered locus names
      MCP_0203

Organism names

Accessions

  • Primary accession
    D1YV03

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer or homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-301Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    345
  • Mass (Da)
    36,943
  • Last updated
    2010-02-09 v1
  • Checksum
    12D9C8F368566230
MADIKKIGILTGGGDCPGLNAVIRAVVFKAGEYGWQVLGVKYGWKGMLNADAIPLTRNDVKDILPLGGTILKTSRTNPYKVEGGEAKVLENAKKMGIDCLVAVGGEDTLGVANKLTKAGLRCVGVPKTIDNDLGATDYTFGYQTAVQIASDAMDRLHTTAKSHDRVLVCEVMGRHAGWMTVDAGMSASAHWIYTPEAKGSVEDCCKMLKERYARGDKYGIVAVAEGAEFSDLDVKAASQTTDSFGHVKLGGVAETLAKEIEKRTGLETRHVVLGHTQRGGSPLAYDRILGTRLGNKAAEMIKNGQFAMMASLRGENVEAVPIEEAVKTLKTVPPQYYDVAKTFFG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP011532
EMBL· GenBank· DDBJ
BAI60275.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp