D1PSV0 · D1PSV0_9BACT
- ProteinEpoxyqueuosine reductase
- GenequeG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids311 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
Catalytic activity
- epoxyqueuosine34 in tRNA + AH2 = queuosine34 in tRNA + A + H2O
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 [4Fe-4S] clusters per monomer.
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 59 | cob(II)alamin (UniProtKB | ChEBI) | |||
Active site | 132 | Proton donor | |||
Binding site | 132 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 153 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 156 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 167 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 186 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 189 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 192 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 196 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 212 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 214 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 239 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 239-240 | cob(II)alamin (UniProtKB | ChEBI) | |||
Binding site | 242 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 246 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 297 | tRNA (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cobalamin binding | |
Molecular Function | epoxyqueuosine reductase activity | |
Molecular Function | metal ion binding | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEpoxyqueuosine reductase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Bacteroidales > Prevotellaceae > Hallella
Accessions
- Primary accessionD1PSV0
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length311
- Mass (Da)35,777
- Last updated2010-02-09 v1
- ChecksumB4EFBC94BD790879
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ACKS01000009 EMBL· GenBank· DDBJ | EFA45468.1 EMBL· GenBank· DDBJ | Genomic DNA |