D1L2X1 · DPOL2_BPK32
- ProteinDepolymerase 2, capsule K21-specific
- Gene38
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids576 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions as a receptor binding protein (RBP) and probably mediates the attachment to the host capsular exopolysaccharides (Probable). Displays a depolymerase activity that specifically degrades the K21-type polysaccharides Klebsiella pneumoniae capsule, which allows the phage to reach the host cell membrane and bind the entry receptor (PubMed:30405575, PubMed:32386574, PubMed:33947754).
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 170 | |||||
Sequence: E | ||||||
Active site | 229 | |||||
Sequence: D | ||||||
Active site | 239 | |||||
Sequence: E | ||||||
Active site | 241 | |||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | virus tail | |
Biological Process | adhesion receptor-mediated virion attachment to host cell | |
Biological Process | symbiont entry into host cell via disruption of host cell envelope | |
Biological Process | symbiont entry into host cell via disruption of host cell glycocalyx |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDepolymerase 2, capsule K21-specific
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Przondovirus > Przondovirus KP32
- Virus hosts
Accessions
- Primary accessionD1L2X1
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 167 | 8 times decreased enzymatic activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 170 | 2000 times decreased enzymatic activity. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 229 | 256 times decreased enzymatic activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 239 | 2000 times decreased enzymatic activity. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 241 | 15000 times decreased enzymatic activity. | ||||
Sequence: D → N |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000458710 | 1-576 | Depolymerase 2, capsule K21-specific | |||
Sequence: MLDNFNQPKGSTIGVLKDGRTIQEAFDSLPRLESFSGSTATDKLRAAITLGVSEVAIGPVEGNGGRPYEFGDVVIPYPLRIVGCGSQGINVTKGTVLKRSAGASFMFHFTGEGQAQRPMGGGLFNINLNGDTATALGDIIKVTQWSYFKANNCAFQNMAGWGIRLKDVMESNISGNLFRRLGGPSGGGILFDDVRSAVTDNVNNLHIEDNTFALMSGPWIGSTANSNPDLIWIVRNKFEFDGTPAAPNTVDSYVLDFQQLSRAFIQDNGFTHFTTERNRYVGVLRVGATAVGTIKFEDNLLFACESAGLIAGGIVVSRGNVNNQGSATTAIKQFTNTSSKLCKLERVINVQSNGNVSVGQQILPDGYINMAELPGNTRLPSEYDADGETTSVLRVPANTQVRQWSVPKMYKDGLTVTKVTVRAKGAAAGAILSLQSGSTVLSTKSIDAGVWKNYVFYVKANQLQETLQLRNTGTADVLADGMVFGKVDYIDWDFAIAPGTLAAGAKYTTPNQSYLDVAGMRVQAVSIPMFDGPTTGLQVWVEATSANGSFVVVMKNDTGSELVTTVTRCRVRAFVS |
Interaction
Subunit
Homotrimer (PubMed:30405575, PubMed:32386574).
The 3 parallel chains are tightly packed together to form an elongated structure of approximately 7 x 12 nm (PubMed:32386574).
Interacts (via N-terminus) with depolymerase 1 (via N-terminus); this interaction probably gives rise to a branched tailspike (PubMed:32386574).
The 3 parallel chains are tightly packed together to form an elongated structure of approximately 7 x 12 nm (PubMed:32386574).
Interacts (via N-terminus) with depolymerase 1 (via N-terminus); this interaction probably gives rise to a branched tailspike (PubMed:32386574).
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 34-360 | Catalytic | ||||
Sequence: SFSGSTATDKLRAAITLGVSEVAIGPVEGNGGRPYEFGDVVIPYPLRIVGCGSQGINVTKGTVLKRSAGASFMFHFTGEGQAQRPMGGGLFNINLNGDTATALGDIIKVTQWSYFKANNCAFQNMAGWGIRLKDVMESNISGNLFRRLGGPSGGGILFDDVRSAVTDNVNNLHIEDNTFALMSGPWIGSTANSNPDLIWIVRNKFEFDGTPAAPNTVDSYVLDFQQLSRAFIQDNGFTHFTTERNRYVGVLRVGATAVGTIKFEDNLLFACESAGLIAGGIVVSRGNVNNQGSATTAIKQFTNTSSKLCKLERVINVQSNGNVSVGQ | ||||||
Region | 380-487 | Carbohydrate binding | ||||
Sequence: PSEYDADGETTSVLRVPANTQVRQWSVPKMYKDGLTVTKVTVRAKGAAAGAILSLQSGSTVLSTKSIDAGVWKNYVFYVKANQLQETLQLRNTGTADVLADGMVFGKV | ||||||
Region | 488-576 | Lectin-like | ||||
Sequence: DYIDWDFAIAPGTLAAGAKYTTPNQSYLDVAGMRVQAVSIPMFDGPTTGLQVWVEATSANGSFVVVMKNDTGSELVTTVTRCRVRAFVS |
Domain
Consists in a catalytic region, a carbohydrate binding region and a lectin-like region (PubMed:32386574).
The active site consists of catalytic dyads Asp-Glu located at the interface between 2 adjacent subunits (PubMed:32386574).
The lectin-like region is probably involved in trimerization (PubMed:32386574).
The active site consists of catalytic dyads Asp-Glu located at the interface between 2 adjacent subunits (PubMed:32386574).
The lectin-like region is probably involved in trimerization (PubMed:32386574).
Sequence similarities
In the N-terminal section; belongs to the Przondovirus depolymerase 2 family.
In the C-terminal section; belongs to the K21-specific depolymerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length576
- Mass (Da)61,687
- Last updated2010-01-19 v1
- ChecksumF57CAAA54812646E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GQ413937 EMBL· GenBank· DDBJ | ACY66700.1 EMBL· GenBank· DDBJ | Genomic DNA |