D1KCD6 · D1KCD6_9GAMM

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1781100200300400500600700
Type
IDPosition(s)Description
Site33Interaction with DNA
Site137Interaction with DNA
Site138Interaction with DNA
Site141Interaction with DNA
Site153Interaction with DNA
Active site282O-(5'-phospho-DNA)-tyrosine intermediate
Site284Interaction with DNA
Site467Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      Sup05_0702

Organism names

Accessions

  • Primary accession
    D1KCD6

Proteomes

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain3-112Toprim
Region161-166Interaction with DNA
Region761-781Disordered

Sequence similarities

Belongs to the type IA topoisomerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    781
  • Mass (Da)
    87,519
  • Last updated
    2010-01-19 v1
  • Checksum
    C7F6B0EAFA6AB1C4
MAKNLVIVESPAKAKTIEKFLGKDYQVKSSIGHIRDMAKKNGIEIENNFKPNYEISADKKKVVADLRKAVKKSEQVYLATDEDREGEAIAWHLLEALDLPKDTPRIVFHEITKGAITKAIQEPRTVNFDVVDAQQARRVIDRLVGFEISGVLWKKVSGAKSAGRVQSPVVRLVVEREREINDHTAKSMFKIKADLVNDAGELIEAKLDKDFATKDEALEFTNALLEAKLNVNSTEKKPSKRSPKAPFITSTLQQEASQKLGFSVKQTMTLAQNLYREGAITYMRTDSFTLSEEAIAAAQKEITSKYGAEYHQARRHKTKDAGAQEAHEAIRPTDLTKPSIPGVEDQVAKLYALIYKRTLASQMSDAKLQKTQIKTNISNRDESFLANGEILVFPGFLSVYDYLSSDDKLLPDLAQGDELTLASFAARESFSRAKPRYTEASLVKKIEEMGIGRPSTFATMVSTVQDRNYVTKETREGVERAYQLIEINKGKVEQSEPTENTGAEKNKLFPTSVAYLLTDFLVKHFGDIVDYKFTANLETDFDTIANQGVVWQGVVKEFYGPFHAKIEAADDISREETHGMRDLGIDPKSGKPVSVRFGRYGAFAQIGHKDDEDKPAFASLRGDLVIETITLDEALELFNMPRTVGETEDFGVIKANYGRFGPYIQYGKKYVSLKEEIPEDVTLEKALELIAAKEKFDAERIIKTFEDSEIQVLNGRFGPYIWNGKKRGKGQKNITIKKFFGDKEPSDLTIEECKKAIAGKVKSKTAKRKKKKPAKKTAKKS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GG729999
EMBL· GenBank· DDBJ
EEZ80231.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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