D1AB74 · LTP2_THECD
- ProteinSteroid side-chain-cleaving aldolase
- Geneltp2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids391 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Probably involved in bile acid degradation (Probable). In vitro, when associated with the ChsH1/ChsH2 hydratase, catalyzes the retroaldol cleavage of 17-hydroxy-3-oxo-4-pregnene-20-carboxyl-CoA (17-HOPC-CoA), forming androst-4-ene-3,17-dione and propionyl-CoA (PubMed:31209106).
The in vivo substrate is probably a closely analogous bile acid degradation metabolite (Probable)
The in vivo substrate is probably a closely analogous bile acid degradation metabolite (Probable)
Catalytic activity
- 17-hydroxy-3-oxochol-4-en-22-oyl-CoA = androst-4-ene-3,17-dione + propanoyl-CoA
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.4 μM | 17-HOPC-CoA | in the presence of ChsH2-DUF35 |
kcat is 7.1 sec-1.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 294 | Proton acceptor | ||||
Sequence: Y | ||||||
Active site | 344 | Proton donor | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity, transferring groups other than amino-acyl groups | |
Molecular Function | lyase activity | |
Biological Process | bile acid catabolic process | |
Biological Process | lipid catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSteroid side-chain-cleaving aldolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Streptosporangiales > Thermomonosporaceae > Thermomonospora
Accessions
- Primary accessionD1AB74
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 82 | Almost loss of activity. | ||||
Sequence: G → P | ||||||
Mutagenesis | 294 | Almost loss of activity. Loss of activity; when associated with F-344. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 296 | 13-fold decrease in catalytic efficiency. | ||||
Sequence: H → A | ||||||
Mutagenesis | 344 | 322-fold decrease in catalytic efficiency. Loss of activity; when associated with F-294. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 346 | 11-fold decrease in catalytic efficiency. | ||||
Sequence: H → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452244 | 1-391 | Steroid side-chain-cleaving aldolase | |||
Sequence: MSVLPGAAAIAGIGATEFSKNSGRSELQLACEAVLAAIADAGLEPSDVDGLVTFTADTSSEIHVARNTGIGELKFFSRVGYGGGAACGTVQQAAMAVATGIAEVVVCYRAFNERSGVRYGLGQAGRQMDQGADSAAYAWLLPFGLNTPAQWVAMFARRYMHEYGATSEDFGRVAVVDRKHAATNPKAWFYQRPITLEDHQNSRWIVEPLHLLDCCQESDGGQALVVVSTERARDLPHPPALIWGAAQGSGYDQHMMTSYYRSEITGIPEMGLVGQQLYAQSGLNPSDIGAAILYDHFTPLVLPQLEELGFCARGEAKDFIADGNLEIGGRLPCNTHGGQLGEAYIHGMNGIAEAVRLVRGTSVNQPGDVTNVLVTAGTGVPTSGLILGADR |
Structure
Sequence
- Sequence statusComplete
- Length391
- Mass (Da)41,404
- Last updated2010-01-19 v1
- ChecksumFEE8D313CF0D8894
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001738 EMBL· GenBank· DDBJ | ACY99017.1 EMBL· GenBank· DDBJ | Genomic DNA |