D0V3Y4 · NMO_PSESP

  • Protein
    Nitronate monooxygenase
  • Gene
    pnoA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. Acts on propionate 3-nitronate (P3N), the presumed physiological substrate. Is likely involved in the degradation of P3N, that allows Pseudomonas sp. JS189 to grow on 3-nitropropionate/P3N as the sole source of nitrogen, carbon, and energy (PubMed:20382807).
Also probably functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism (By similarity).
Is not active against 3-nitropropionate, nor with neutral or nitronate forms of 1-nitropropane, 2-nitropropane, nitroethane, nitromethane, 1-nitropentane, and 2-nitroethanol (PubMed:20382807).

Miscellaneous

At physiological pH, 3-nitropropionate (3-NPA) exists in equilibrium with its conjugate base, propionate-3-nitronate (P3N).
P3N is a potent irreversible inhibitor of the key enzyme succinate dehydrogenase in the Krebs cycle and electron transport chain. P3N has been shown to be a toxic metabolite to bacteria, plants, fungi, mammals or any organism that uses succinate dehydrogenase.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
30 μMpropionate 3-nitronate

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site69FMN (UniProtKB | ChEBI)
Binding site176FMN (UniProtKB | ChEBI)
Binding site181FMN (UniProtKB | ChEBI)
Binding site217FMN (UniProtKB | ChEBI)
Binding site236-239FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionnitronate monooxygenase activity
Molecular Functionnucleotide binding
Biological Processresponse to toxic substance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitronate monooxygenase
  • EC number
  • Short names
    NMO
  • Alternative names
    • Propionate 3-nitronate monooxygenase
      (P3N monooxygenase
      )

Gene names

    • Name
      pnoA

Organism names

  • Taxonomic identifier
  • Strain
    • JS189
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    D0V3Y4

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004454231-349Nitronate monooxygenase

Expression

Induction

Constitutively expressed.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    349
  • Mass (Da)
    36,457
  • Last updated
    2009-12-15 v1
  • Checksum
    E01472F50366F6FE
MSNSLLSLLNIELPIIQSPMVGVSTPRLAAAVSNAGGLGSIGIGASNVEQARAMLRETAALTDRPFNVNLFCHVPARADAAREAQWLAFLAPLFAEFESPAPAALREIYTSFVEDFAMLEMLLEEKPAVVSFHFGLPPQSSIDALKDAGIVLLACVTNLAEAQQAEHAGVHALVAQGYEAGGHRGVFDPQQDSEMGTFALVRVLTDACQLPVIAAGGIMDGAGIKAVMQLGASAAQLGTAFILCPESSANPAYRDALQGPRAHQTRVTSAISGRPARGMVNRNYIDLETNAPALPDYPIAYDANKALNAAAANKANTDFAVQWAGQGAPLARSMPAAALVNLLAAEMKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GU014557
EMBL· GenBank· DDBJ
ACX83564.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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