D0R1W2 · D0R1W2_LACJF

Function

function

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 divalent ions per subunit.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site17ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site19Mg2+ (UniProtKB | ChEBI)
Binding site45K+ (UniProtKB | ChEBI)
Binding site58L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site101L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site177-179ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site244-245ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site253ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site253L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site259-260ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site276ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site280ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site284L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionmethionine adenosyltransferase activity
Biological Processone-carbon metabolic process
Biological ProcessS-adenosylmethionine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    S-adenosylmethionine synthase
  • EC number
  • Short names
    AdoMet synthase
  • Alternative names
    • MAT
    • Methionine adenosyltransferase

Gene names

    • Name
      metK
    • Ordered locus names
      FI9785_519

Organism names

Accessions

  • Primary accession
    D0R1W2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer; dimer of dimers.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-20Disordered
Domain6-102S-adenosylmethionine synthetase N-terminal
Region101-111Flexible loop
Domain127-245S-adenosylmethionine synthetase central
Domain247-385S-adenosylmethionine synthetase C-terminal

Sequence similarities

Belongs to the AdoMet synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    402
  • Mass (Da)
    43,994
  • Last updated
    2009-12-15 v1
  • Checksum
    CB34F965A796ACE9
MKKEKHLFTSESVSEGHPDKVADQISDAILDAILAKDPDGRVACETTVTTGLVLVVGEISTSAYVDIQSVVRKTILEIGYNRPELGFDGNNCAILVDIDEQSSDIAGGVNESLETRENHQDKDDLDKIGAGDQGLMFGFAIKETPELMPLPISLAHSLMRRVASLRKEGRLDWLRPDAKAQVTVEYDDENKPKRVDTVVISTQTDAIVTNDEICEAMIDMVIKKVIPSQYLDKDTKFLINPSGRFVIGGPKGDSGLTGRKIIVDTYGGYARHGGGAFSGKDLTKVDRSASYAARYVAKNIVAAGLAYQCEIQLAYAIGVAHPVSIMVDTHGTSKVSEDLLVESVRNVFDLRPAGIIEMLNLKRPIYRQTAAYGHFGRTDVDLPWEHTDKVEALKTYVSEHAE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN298497
EMBL· GenBank· DDBJ
CAX66399.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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