D0L1K6 · D0L1K6_HALNC

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site303Mg2+ (UniProtKB | ChEBI); catalytic
Binding site346Mg2+ (UniProtKB | ChEBI); catalytic
Binding site404Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site407Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • Ordered locus names
      Hneap_1756

Organism names

Accessions

  • Primary accession
    D0L1K6

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-120S1 motif
Region404-407Required for zinc-mediated homotetramerization and catalytic activity
Region518-538Disordered
Compositional bias584-611Basic and acidic residues
Region584-720Disordered
Compositional bias623-648Basic and acidic residues
Compositional bias665-681Polar residues
Compositional bias691-709Basic and acidic residues
Region895-936Disordered
Region957-1016Disordered
Compositional bias977-991Polar residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,016
  • Mass (Da)
    109,742
  • Last updated
    2009-12-15 v1
  • Checksum
    42771660CDDE48CA
MKRMLINATQAEEIRVALVDGQTLYDIDIETPGHEQKKSNVYKALITRIEPSLEAVFVNYGADRHGFLPFKEIARSYFDSEAVDDKGRPIIKSALKEGQEIIVQVEKEERGNKGAALTTFVSLAGRYLVLMPNNPRAGGVSRRIEGDDRAEIKAALSELVVPEGMGLIVRTAGVGREPAELQSDLDYLQQVWDAIVKKSEARKAPFLIYQESDLIIRALRDYMRNDIGEVLIDEPNMHQQALDFVNLVSPDSVDKIRLYQDRTPLFTRYQIESQIESAYERNVTLPSGGELVFDVAEALTAVDINSGRSTKGQDIEDTAFNTNLEAADEIARQLRLRDLGGLVVIDFIDMGQPKHQREVENRLRDALKMDRARVQLGRISRFGLLEMSRQRLSASLEESAQHVCPRCMGQGHIRSVESLALSIMRLMIDESMKDQTGRVVAQVPVDVGTYLLNEKRLQLHEIEQNHQVEVLVIPNINLETPHFKIERVRRSDLAESPANSRALIEPLAVAVPDLERAPRRPAAPVEPIRPVPQSAPRPKVVASVPDVATEPAASTSAANIGALGLLKRVIGQIFGAGDKRVEAEVAAPRDPARKASSNRPPRAKDENRATPNRLPRAATQTSESEARLEDKRRDEGKVEGTKPEGSSNRRRRSRSRKPAEGQRDAQVNTAASANQPAEAPAVTTDTAEGKGSAKAPEKTTARQREPRSSEPAVRPIAAHEQSLIDQQLKAPVASPSALPLNVAVKRDPAAALAPNAALGLLADDVIVPALVAAAITPKAEAQIAAPQPEDTAANEVNVAPSAPMVESQAEAPTDTTIAAVTPVVAEPVSAAEPVIHTKEEMPSVAATESPVETSTLIPEAIAEQIVAQPVEATNDSPWSQASAILAAKQDEVAAPAASETKVADEPVSEKAPTDVQPNASAVDEVEPPVAQAESIHDSPAMASEHAPVAEAIIESAPVESEPVEAVTEAEQPEATTADAVTEATTQVETAPQEDVHPEAIATTDSAEETVRKEPNA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias584-611Basic and acidic residues
Compositional bias623-648Basic and acidic residues
Compositional bias665-681Polar residues
Compositional bias691-709Basic and acidic residues
Compositional bias977-991Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001801
EMBL· GenBank· DDBJ
ACX96579.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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