D0KWN0 · D0KWN0_HALNC

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site45-49NAD+ (UniProtKB | ChEBI)
Binding site95-96NAD+ (UniProtKB | ChEBI)
Binding site127NAD+ (UniProtKB | ChEBI)
Active site129N6-AMP-lysine intermediate
Binding site150NAD+ (UniProtKB | ChEBI)
Binding site188NAD+ (UniProtKB | ChEBI)
Binding site305NAD+ (UniProtKB | ChEBI)
Binding site329NAD+ (UniProtKB | ChEBI)
Binding site423Zn2+ (UniProtKB | ChEBI)
Binding site426Zn2+ (UniProtKB | ChEBI)
Binding site447Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • Ordered locus names
      Hneap_0163

Organism names

Accessions

  • Primary accession
    D0KWN0

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain606-685BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    685
  • Mass (Da)
    74,909
  • Last updated
    2009-12-15 v1
  • Checksum
    543C540751686111
MASSLPDLFSAPSEDGDRARLLALRDEIERHNFRYYVLDDPEISDAAFDRLMRELQQIESAHPEWITPDSPTQRVGSPTFTTRFAPVTHHQAMLSLDNVFSVEEWDAFTQRIQDRLKRDAPLVFAAEPKFDGLAINLIYRQGQLVQAATRGDGQTGEDVTLNVRTIAAIPQQLPDSLLSCDLLEVRGEIVMSHKAFAALNAAADARGDKRFVNPRNAAAGSLRQKDPRITAKRQLQFFAYGVGAVDGADLPDSQLGLLDWLADLGFTVSEWRRRCEGRDEVLAYQQEMSARRSQLDYDIDGVVFKINAQSEQDALGFVAKAPRWAIAFKFPAEEATSRVHAVDFQVGRTGALTPVARIDPVFVGGVTVSNITLHNIDEITRKDIRIGDTVVVRRAGDVIPEVVRVLPELRPADARLVALPATCPVCGSEVVRAEGEAIARCSGGLFCPAQRREAIKHFASRKAMNIDGLGEKWIELLLEHKLVEHVDDLFHLTVPQVLTLPRMGEKSAQNLVDALSAAKQTTLPRFLYALGIREVGEVTAAGLAAHFRNLDALNAATLEDLQAVPDVGPVVAQHVFTFLRQPHNQEVIRNLIDAGVHWPAIVATDANALPLAGRTYVLTGTLVGMSREEAGERLKALGAKVSGSVSSKTTAVIAGSEAGSKLTKANDLGVPVLDETDLQKLLRNL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001801
EMBL· GenBank· DDBJ
ACX95027.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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