D0EZM8 · NS1_HBOC1
- ProteinInitiator protein NS1
- GeneNS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids781 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. The helicase activity unwinds DNA in a 3'-5' direction on the longer strand. Participates in the transcriptional regulation of several promoters.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: The endonuclease active site can probably bind other divalent cations.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 108 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 115 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 117 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 211 | For nuclease activity | ||||
Sequence: Y | ||||||
Binding site | 423-430 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GPASTGKT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA replication | |
Biological Process | viral DNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInitiator protein NS1
- EC number
- Short namesNS1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Quintoviricetes > Piccovirales > Parvoviridae > Parvovirinae > Bocaparvovirus > Bocaparvovirus primate1
- Virus hosts
Accessions
- Primary accessionD0EZM8
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 384 | in strain: Eg/BSU-1 | ||||
Sequence: D → N | ||||||
Natural variant | 398 | in strain: Eg/BSU-1 | ||||
Sequence: P → L | ||||||
Natural variant | 536 | in strain: Eg/BSU-1 | ||||
Sequence: Q → H | ||||||
Natural variant | 566 | in strain: Eg/BSU-1 | ||||
Sequence: Y → H | ||||||
Natural variant | 639 | in strain: Eg/BSU-1 | ||||
Sequence: D → K | ||||||
Natural variant | 649 | in strain: KU3 and Salvator1 | ||||
Sequence: E → K | ||||||
Natural variant | 746 | in strain: KU3 and Salvator1 | ||||
Sequence: F → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 7 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445633 | 1-781 | Initiator protein NS1 | |||
Sequence: MAFNPPVIRAFSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLEDRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGLILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKNYLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPGPQTMAYGGRGPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCKELNLLVYEDLVANCPELLLMLEGQPGGARLIEQVLGMHHINVCSNFTALTYLFHLHPVTSLDSDNKALQLLLIQGYNPLAVGHALCCVLNKQFGKQNTVCFYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRIDVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITATEIAALLQWCFNEYDCTLTGFKQKWNLDKIPNSFPLGVLCPTHSQDFTLHENGYCTDCGGYLPHSADNSMYTDRASETSTGDITPSDLGDSDGEDTEPETSQVDYCPPKKRRLTAPASPPNSPASSVSTITFFNTWHAQPRDEDELREYERQASLLQKKRESRKRGEEETLADNSSQEQEPQPDPTQWGERLGFISSGTPNQPPIVLHCFEDLRPSDEDEGEYIGEKRQ |
Keywords
- PTM
Interaction
Subunit
Homooligomer; when bound to DNA.
Structure
Family & Domains
Features
Showing features for domain, motif, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 11-271 | PV NS1-Nuc | ||||
Sequence: FSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLEDRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGLILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKNYLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPG | ||||||
Motif | 115-117 | RCR-2 | ||||
Sequence: HCH | ||||||
Region | 193-197 | Ori-binding | ||||
Sequence: RRGDL | ||||||
Motif | 211-215 | RCR-3 | ||||
Sequence: YLLPK | ||||||
Domain | 397-552 | SF3 helicase | ||||
Sequence: NPLAVGHALCCVLNKQFGKQNTVCFYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRIDVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITA | ||||||
Region | 606-781 | Transactivation | ||||
Sequence: YCTDCGGYLPHSADNSMYTDRASETSTGDITPSDLGDSDGEDTEPETSQVDYCPPKKRRLTAPASPPNSPASSVSTITFFNTWHAQPRDEDELREYERQASLLQKKRESRKRGEEETLADNSSQEQEPQPDPTQWGERLGFISSGTPNQPPIVLHCFEDLRPSDEDEGEYIGEKRQ | ||||||
Compositional bias | 621-638 | Polar residues | ||||
Sequence: SMYTDRASETSTGDITPS | ||||||
Region | 621-679 | Disordered | ||||
Sequence: SMYTDRASETSTGDITPSDLGDSDGEDTEPETSQVDYCPPKKRRLTAPASPPNSPASSV | ||||||
Compositional bias | 709-724 | Basic and acidic residues | ||||
Sequence: QKKRESRKRGEEETLA | ||||||
Region | 709-781 | Disordered | ||||
Sequence: QKKRESRKRGEEETLADNSSQEQEPQPDPTQWGERLGFISSGTPNQPPIVLHCFEDLRPSDEDEGEYIGEKRQ | ||||||
Compositional bias | 763-781 | Basic and acidic residues | ||||
Sequence: EDLRPSDEDEGEYIGEKRQ |
Domain
In the N-terminus, the endonuclease region is involved in binding to the origin of replication (PubMed:23966383).
In the middle, there are the ATPase and helicase activities (By similarity).
The C-terminus probably contains a transactivation domain (By similarity).
In the middle, there are the ATPase and helicase activities (By similarity).
The C-terminus probably contains a transactivation domain (By similarity).
Sequence similarities
Belongs to the parvoviruses initiator protein NS1 family.
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 5 isoforms produced by Alternative splicing.
D0EZM8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameNS1
- Length781
- Mass (Da)88,180
- Last updated2009-11-24 v1
- ChecksumBF1D92C6BF0562DE
D0EZM8-2
- NameNS1-70
D0EZM8-3
- NameNS2
- Differences from canonical
- 289-597: Missing
D0EZM8-4
- NameNS3
- Differences from canonical
- 1-274: Missing
D0EZM8-5
- NameNS4
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_059924 | 1-274 | in isoform NS3 and isoform NS4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_059925 | 289-597 | in isoform NS2 and isoform NS4 | |||
Sequence: Missing | ||||||
Compositional bias | 621-638 | Polar residues | ||||
Sequence: SMYTDRASETSTGDITPS | ||||||
Alternative sequence | VSP_059926 | 639 | in isoform NS1-70 | |||
Sequence: D → K | ||||||
Alternative sequence | VSP_059927 | 640-781 | in isoform NS1-70 | |||
Sequence: Missing | ||||||
Compositional bias | 709-724 | Basic and acidic residues | ||||
Sequence: QKKRESRKRGEEETLA | ||||||
Compositional bias | 763-781 | Basic and acidic residues | ||||
Sequence: EDLRPSDEDEGEYIGEKRQ |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ000496 EMBL· GenBank· DDBJ | AAY45700.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
GQ925675 EMBL· GenBank· DDBJ | ACX50492.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
GQ925675 EMBL· GenBank· DDBJ | ACX50493.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JQ411251 EMBL· GenBank· DDBJ | AFC37600.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JQ411251 EMBL· GenBank· DDBJ | AFC37603.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JQ923422 EMBL· GenBank· DDBJ | AFR53039.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JQ923422 EMBL· GenBank· DDBJ | AFR53040.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KU557404 EMBL· GenBank· DDBJ | AND46431.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KJ634207 EMBL· GenBank· DDBJ | AIC76457.1 EMBL· GenBank· DDBJ | Genomic DNA |