D0EZM8 · NS1_HBOC1

Function

function

Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. The helicase activity unwinds DNA in a 3'-5' direction on the longer strand. Participates in the transcriptional regulation of several promoters.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: The endonuclease active site can probably bind other divalent cations.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site108a divalent metal cation (UniProtKB | ChEBI)
Binding site115a divalent metal cation (UniProtKB | ChEBI)
Binding site117a divalent metal cation (UniProtKB | ChEBI)
Active site211For nuclease activity
Binding site423-430ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular Functionendonuclease activity
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Biological ProcessDNA replication
Biological Processviral DNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Initiator protein NS1
  • EC number
  • Short names
    NS1
  • Alternative names
    • Non-structural protein 1
    • Non-structural protein NS1

Gene names

    • Name
      NS1

Organism names

Accessions

  • Primary accession
    D0EZM8
  • Secondary accessions
    • A0A068B244
    • A0A1L2DWK9
    • A0A384KU82
    • H9CWJ3
    • J9RYC4

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant384in strain: Eg/BSU-1
Natural variant398in strain: Eg/BSU-1
Natural variant536in strain: Eg/BSU-1
Natural variant566in strain: Eg/BSU-1
Natural variant639in strain: Eg/BSU-1
Natural variant649in strain: KU3 and Salvator1
Natural variant746in strain: KU3 and Salvator1

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 7 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004456331-781Initiator protein NS1

Keywords

Interaction

Subunit

Homooligomer; when bound to DNA.

Structure

Family & Domains

Features

Showing features for domain, motif, region, compositional bias.

TypeIDPosition(s)Description
Domain11-271PV NS1-Nuc
Motif115-117RCR-2
Region193-197Ori-binding
Motif211-215RCR-3
Domain397-552SF3 helicase
Region606-781Transactivation
Compositional bias621-638Polar residues
Region621-679Disordered
Compositional bias709-724Basic and acidic residues
Region709-781Disordered
Compositional bias763-781Basic and acidic residues

Domain

In the N-terminus, the endonuclease region is involved in binding to the origin of replication (PubMed:23966383).
In the middle, there are the ATPase and helicase activities (By similarity).
The C-terminus probably contains a transactivation domain (By similarity).

Sequence similarities

Family and domain databases

Sequence & Isoforms

Align isoforms (5)
  • Sequence status
    Complete

This entry describes 5 isoforms produced by Alternative splicing.

D0EZM8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    NS1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    781
  • Mass (Da)
    88,180
  • Last updated
    2009-11-24 v1
  • Checksum
    BF1D92C6BF0562DE
MAFNPPVIRAFSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLEDRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGLILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKNYLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPGPQTMAYGGRGPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCKELNLLVYEDLVANCPELLLMLEGQPGGARLIEQVLGMHHINVCSNFTALTYLFHLHPVTSLDSDNKALQLLLIQGYNPLAVGHALCCVLNKQFGKQNTVCFYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRIDVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITATEIAALLQWCFNEYDCTLTGFKQKWNLDKIPNSFPLGVLCPTHSQDFTLHENGYCTDCGGYLPHSADNSMYTDRASETSTGDITPSDLGDSDGEDTEPETSQVDYCPPKKRRLTAPASPPNSPASSVSTITFFNTWHAQPRDEDELREYERQASLLQKKRESRKRGEEETLADNSSQEQEPQPDPTQWGERLGFISSGTPNQPPIVLHCFEDLRPSDEDEGEYIGEKRQ

D0EZM8-2

D0EZM8-3

D0EZM8-4

  • Name
    NS3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

D0EZM8-5

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0599241-274in isoform NS3 and isoform NS4
Alternative sequenceVSP_059925289-597in isoform NS2 and isoform NS4
Compositional bias621-638Polar residues
Alternative sequenceVSP_059926639in isoform NS1-70
Alternative sequenceVSP_059927640-781in isoform NS1-70
Compositional bias709-724Basic and acidic residues
Compositional bias763-781Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ000496
EMBL· GenBank· DDBJ
AAY45700.1
EMBL· GenBank· DDBJ
Genomic DNA
GQ925675
EMBL· GenBank· DDBJ
ACX50492.1
EMBL· GenBank· DDBJ
Genomic DNA
GQ925675
EMBL· GenBank· DDBJ
ACX50493.1
EMBL· GenBank· DDBJ
Genomic DNA
JQ411251
EMBL· GenBank· DDBJ
AFC37600.1
EMBL· GenBank· DDBJ
Genomic DNA
JQ411251
EMBL· GenBank· DDBJ
AFC37603.1
EMBL· GenBank· DDBJ
Genomic DNA
JQ923422
EMBL· GenBank· DDBJ
AFR53039.1
EMBL· GenBank· DDBJ
Genomic DNA
JQ923422
EMBL· GenBank· DDBJ
AFR53040.1
EMBL· GenBank· DDBJ
Genomic DNA
KU557404
EMBL· GenBank· DDBJ
AND46431.1
EMBL· GenBank· DDBJ
Genomic DNA
KJ634207
EMBL· GenBank· DDBJ
AIC76457.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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