C9X0M5 · RNC_NEIM8

Function

function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracRNA are required for cleavage of invading DNA. Involved in 3'-end processing but not 5'-end processing of crRNA and tracrRNA (PubMed:23706818).

Catalytic activity

  • Endonucleolytic cleavage to 5'-phosphomonoester.
    EC:3.1.26.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site46Mg2+ (UniProtKB | ChEBI)
Active site50
Binding site119Mg2+ (UniProtKB | ChEBI)
Binding site122Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmetal ion binding
Molecular Functionribonuclease III activity
Molecular FunctionrRNA binding
Biological ProcessmRNA processing
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease 3
  • EC number
  • Alternative names
    • Ribonuclease III

Gene names

    • Name
      rnc
    • Ordered locus names
      NMV_1713

Organism names

Accessions

  • Primary accession
    C9X0M5

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Altered processing of 3' end of CRISPR crRNA and tracrRNA, but no effect on CRISPR interference during plasmid transformation.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004299901-239Ribonuclease 3

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-133RNase III
Domain160-230DRBM

Sequence similarities

Belongs to the ribonuclease III family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    239
  • Mass (Da)
    26,853
  • Last updated
    2009-11-24 v1
  • Checksum
    54D0F0473049607C
MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNERFEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLGAGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKDGKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQEAAKEALKWLEEKLPLKRKKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FM999788
EMBL· GenBank· DDBJ
CAX50526.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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