C9X0M5 · RNC_NEIM8
- ProteinRibonuclease 3
- Genernc
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids239 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracRNA are required for cleavage of invading DNA. Involved in 3'-end processing but not 5'-end processing of crRNA and tracrRNA (PubMed:23706818).
Catalytic activity
Cofactor
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding | |
Molecular Function | ribonuclease III activity | |
Molecular Function | rRNA binding | |
Biological Process | mRNA processing | |
Biological Process | rRNA processing | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease 3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionC9X0M5
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Altered processing of 3' end of CRISPR crRNA and tracrRNA, but no effect on CRISPR interference during plasmid transformation.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429990 | 1-239 | Ribonuclease 3 | |||
Sequence: MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNERFEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLGAGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKDGKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQEAAKEALKWLEEKLPLKRKKK |
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 11-133 | RNase III | ||||
Sequence: HAAIQKKLGYAFRDISLLRQALTHRSHHAKHNERFEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLGAGELKSGGFRRPSILADAMEAMFAAVSFDAD | ||||||
Domain | 160-230 | DRBM | ||||
Sequence: DGKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQEAAKEALKWLEE |
Sequence similarities
Belongs to the ribonuclease III family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length239
- Mass (Da)26,853
- Last updated2009-11-24 v1
- Checksum54D0F0473049607C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FM999788 EMBL· GenBank· DDBJ | CAX50526.1 EMBL· GenBank· DDBJ | Genomic DNA |