C9DSS4 · C9DSS4_CVTKE

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5551
Active site5553
Active site5650
Active site5726
Active site5731
Binding site5789-5795S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6204
Active site6219
Active site6259

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

Accessions

  • Primary accession
    C9DSS4

Proteomes

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1746-1762Helical
Transmembrane1843-1870Helical
Transmembrane1882-1907Helical
Transmembrane2280-2298Helical
Transmembrane2555-2577Helical
Transmembrane2583-2602Helical
Transmembrane2609-2627Helical
Transmembrane2639-2672Helical
Transmembrane3094-3114Helical
Transmembrane3120-3137Helical
Transmembrane3149-3171Helical
Transmembrane3191-3208Helical
Transmembrane3215-3234Helical
Transmembrane3254-3277Helical
Transmembrane3289-3316Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1801↔1807

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain674-779Ubiquitin-like
Domain1004-1179Macro
Domain1175-1227Ubiquitin-like
Domain1236-1497Peptidase C16
Domain1768-18323Ecto
Region1910-2000Y1
Domain1910-2262CoV Nsp3 Y
Region1914-1927ZF1
Region1960-1970ZF2
Region2001-2262CoV-Y
Region2163-2262Y4
Domain2681-2776Nsp4C
Domain2777-3083Peptidase C30
Domain3376-3458RdRp Nsp7 cofactor
Domain3459-3668RdRp Nsp8 cofactor
Domain3669-3779Nsp9 ssRNA-binding
Domain3781-3922ExoN/MTase coactivator
Domain3936-4194NiRAN
Domain4199-4297Nsp12 Interface
Domain4298-4864Nsp12 RNA-dependent RNA polymerase
Domain4544-4706RdRp catalytic
Domain4865-4947CV ZBD
Domain5119-5471+RNA virus helicase C-terminal
Domain5533-5745ExoN
Domain5754-5981N7-MTase
Region5869-5883GpppA-binding
Domain5982-6042Nsp15 N-terminal oligomerization
Domain6043-6158AV-Nsp11N/CoV-Nsp15M
Domain6175-6316NendoU
Domain6319-6596Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,596
  • Mass (Da)
    739,957
  • Last updated
    2009-11-03 v1
  • Checksum
    75CE8DE790713099

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GQ427173
EMBL· GenBank· DDBJ
ACV87243.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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