C8VQY7 · YPT7_EMENI
- ProteinYpt/Rab-type GTPase avaA
- GeneavaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids205 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion (By similarity).
AvaA functions in vacuolar biogenesis (PubMed:12095681).
AvaA functions in vacuolar biogenesis (PubMed:12095681).
Activity regulation
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17-23 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SGVGKTS | ||||||
Binding site | 33-40 | GTP (UniProtKB | ChEBI) | ||||
Sequence: FSGSYKAT | ||||||
Binding site | 66 | GTP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 125-128 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKID | ||||||
Binding site | 157-159 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SAK |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | fungal-type vacuole membrane | |
Cellular Component | late endosome | |
Cellular Component | vacuolar membrane | |
Cellular Component | vacuole | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | endosome organization | |
Biological Process | regulation of vacuole fusion, non-autophagic | |
Biological Process | vacuole fusion, non-autophagic |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameYpt/Rab-type GTPase avaA
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionC8VQY7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Displays highly fragmented vacuoles.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 22 | Constitutively in the GDP-bound conformation. Causes loss of function during vacuolar morphogenesis. | ||||
Sequence: T → N | ||||||
Mutagenesis | 67 | Constitutively in the GTP-bound conformation. Promotes vacuolar fusion resulting in large swollen vacuoles. | ||||
Sequence: Q → L |
PTM/Processing
Features
Showing features for chain, lipidation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451056 | 1-205 | Ypt/Rab-type GTPase avaA | |||
Sequence: MSSRKKVMLKVIILGDSGVGKTSLMNQYVNKKFSGSYKATIGADFLTKEVLVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNNSKSFEALDSWRDEFLIQASPRDPESFPFVVIGNKIDMEESKRMISSKRAMTFCQSKGNIPYFETSAKEAVNVEQAFEVIARSALAQEEAEEYGGDYTDPINIHDTTERDGCAC | ||||||
Lipidation | 203 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 205 | Cysteine methyl ester | ||||
Sequence: C | ||||||
Lipidation | 205 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 37-45 | Effector region | ||||
Sequence: YKATIGADF |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length205
- Mass (Da)22,941
- Last updated2009-11-03 v1
- Checksum0E927E2D76F6BB32
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB072431 EMBL· GenBank· DDBJ | BAB88682.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BN001308 EMBL· GenBank· DDBJ | CBF90222.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACD01000003 EMBL· GenBank· DDBJ | EAA65267.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |