C7U0T4 · C7U0T4_9REOV

Function

function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca2+ concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors.

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.
Some rotavirus strains are neuraminidase-sensitive and require sialic acid to attach to the cell surface. Some rotavirus strains are integrin-dependent. Some rotavirus strains depend on ganglioside for their entry into the host cell. Hsp70 also seems to be involved in the entry of some strains.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site98Ca2+ 1 (UniProtKB | ChEBI)
Binding site181Ca2+ 2 (UniProtKB | ChEBI)
Binding site210Ca2+ 1 (UniProtKB | ChEBI)
Binding site218Ca2+ 1 (UniProtKB | ChEBI)
Binding site220Ca2+ 1 (UniProtKB | ChEBI)
Binding site232Ca2+ 2 (UniProtKB | ChEBI)
Binding site233Ca2+ 2 (UniProtKB | ChEBI)
Binding site235Ca2+ 2 (UniProtKB | ChEBI)
Binding site305Ca2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum lumen
Cellular Componentmembrane
Cellular ComponentT=13 icosahedral viral capsid
Cellular Componentviral outer capsid
Molecular Functionmetal ion binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Outer capsid glycoprotein VP7

Gene names

    • Name
      VP7

Organism names

  • Taxonomic identifier
  • Strain
    • Phea14246-Hun-08
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus > Rotavirus A

Accessions

  • Primary accession
    C7U0T4

Subcellular Location

Virion
Note: The outer layer contains 780 copies of VP7, grouped as 260 trimers. Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane9-27Helical
Transmembrane33-49Helical

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

Type
IDPosition(s)Description
ChainPRO_502353821354-330Outer capsid glycoprotein VP7
Disulfide bond85↔138
Disulfide bond169↔253
Disulfide bond195↔248
Disulfide bond200↔211

Post-translational modification

N-glycosylated.
The N-terminus is blocked possibly by pyroglutamic acid.

Keywords

Interaction

Subunit

Homotrimer. 2 Ca2+ ions bound at each subunit interface in the trimer hold the trimer together.
Homotrimer; disulfide-linked. 2 Ca2+ ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region169-171CNP motif; interaction with ITGAV/ITGB3

Sequence similarities

Belongs to the rotavirus VP7 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    330
  • Mass (Da)
    37,547
  • Last updated
    2009-10-13 v1
  • Checksum
    9361C1990FEEE7DB
MYSTECTSFFLEIIFYVILYTVVSSILERMSQLVNWILIFIIFFSLCVSKCSSQNFGINVPITGSLDVTVLNSTQTQIGLTSTLCIYYPKEASDEIKDSEWKNTVVQLLLTKGWPTTSVYLNEYNDLAAFSNDPKLYCDYNIVLVKYTDSNVALDISELAELLLYEWLCNPMDVTLYYYQQTSESNKWIAMGTNCTIKVCPLNAQTLGIGCQTTNVNTFEQVTASEQLAIIDVVDGVNHKIDYTSNTCTIKNCVRLNPRTNVAIIQIGGPEILDISEDPMVVPKMARMTRINWKKWWQVFYTIVDYIDTIVNAMSKRATSLDASSYYFRV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN393054
EMBL· GenBank· DDBJ
CAY77576.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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