C7F6X3 · IBP_LEUSY

Function

function

Confers freeze tolerance. Binds to the surface of ice crystals and inhibits their growth. Has low thermal hysteresis (TH) activity, which is the ability to lower the freezing point of an aqueous solution below its melting point (PubMed:20067781, PubMed:22303017, PubMed:22426061, PubMed:22622645, PubMed:23203635, PubMed:24699650).
The TH activity of this protein is approximately 0.2 degrees Celsius at 50 uM and 0.3 degrees Celsius at 400 uM (PubMed:24699650).

Biotechnology

Improves cryopreservation of the human red blood cells (RBCs). Almost 90 % hemolysis damage induced to RBCs by freeze thawing with the slow warming at 22 degrees Celsius is dramatically decreased to less than 16 % when 0.4 or 0.8 mg/ml of this protein is used as a cryoprotectant. The post-thaw cell counts of RBCs frozen in the presence of 0.8 mg/ml of this protein and thawed by slow warming at 22 degrees Celsius is almost the same as that of non-frozen intact RBCs, whereas the recovery of RBCs frozen in its absence is less than 30 %.

Features

Showing features for site.

126120406080100120140160180200220240260
TypeIDPosition(s)Description
Site65Ice-binding
Site147Ice-binding
Site234Ice-binding

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionice binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Ice-binding protein
  • Alternative names
    • Antifreeze protein
      (AFP
      )
    • LeIBP

Gene names

    • Name
      AFP

Organism names

Accessions

  • Primary accession
    C7F6X3

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis43Has 35% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis49Has 89% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis65Has 28% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis109Has 92% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis147Has 62% thermal hysteresis (TH) activity of that of the wild-type. Loss of TH activity; when associated with Y-234.
Mutagenesis171Has 67% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis198Has 50% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis216Has 59% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis222Has 64% thermal hysteresis (TH) activity of that of the wild-type.
Mutagenesis234Has 47% thermal hysteresis (TH) activity of that of the wild-type. Loss of TH activity; when associated with Y-147.
Mutagenesis239No effect on thermal hysteresis (TH) activity.
Mutagenesis244-261Has 13% higher thermal hysteresis (TH) activity compared to the wild-type. Loss of homodimerization.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-20
ChainPRO_501108893321-261Ice-binding protein
Glycosylation185N-linked (GlcNAc...) asparagine

Post-translational modification

Glycosylated (PubMed:20067781, PubMed:22303017, PubMed:22426061, PubMed:23203635).
Glycosylation is not required for the thermal hysteresis (TH) activity (PubMed:22426061).
Glycosylation may increase stability and secretion of this protein (Probable)

Keywords

PTM databases

Interaction

Subunit

Homodimer (PubMed:22303017, PubMed:22426061, PubMed:24699650).
Dimerization is not required for the thermal hysteresis (TH) activity (PubMed:22303017).

Family & Domains

Sequence similarities

Belongs to the ice-binding protein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    261
  • Mass (Da)
    26,807
  • Last updated
    2009-09-22 v1
  • Checksum
    F114BC33CBC9B469
MSLLSIITIGLAGLGGLVNGQRDLSVELGVASNFAILAKAGISSVPDSAILGDIGVSPAAATYITGFGLTQDSSTTYATSPQVTGLIYAADYSTPTPNYLAAAVANAETAYNQAAGFVDPDFLELGAGELRDQTLVPGLYKWTSSVSVPTDLTFEGNGDATWVFQIAGGLSLADGVAFTLAGGANSTNIAFQVGDDVTVGKGAHFEGVLLAKRFVTLQTGSSLNGRVLSQTEVALQKATVNSPFVPAPEVVQKRSNARQWL

Mass Spectrometry

Molecular mass is 25,565 Da. Determined by MALDI. The measured mass is that of the mature protein.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GQ336994
EMBL· GenBank· DDBJ
ACU30806.1
EMBL· GenBank· DDBJ
Genomic DNA
GQ336995
EMBL· GenBank· DDBJ
ACU30807.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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