C6E6N2 · PYRDB_GEOSM
- ProteinDihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
- GenepyrD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids305 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of dihydroorotate to orotate with NAD+ as electron acceptor.
Catalytic activity
- (S)-dihydroorotate + NAD+ = orotate + NADH + H+
Cofactor
Note: Binds 1 FMN per subunit.
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD+ route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23 | FMN (UniProtKB | ChEBI) | |||
Binding site | 47 | substrate | |||
Binding site | 47-48 | FMN (UniProtKB | ChEBI) | |||
Binding site | 71-75 | substrate | |||
Binding site | 101 | FMN (UniProtKB | ChEBI) | |||
Binding site | 129 | FMN (UniProtKB | ChEBI) | |||
Binding site | 129 | substrate | |||
Active site | 132 | Nucleophile | |||
Binding site | 167 | FMN (UniProtKB | ChEBI) | |||
Binding site | 193 | FMN (UniProtKB | ChEBI) | |||
Binding site | 194-195 | substrate | |||
Binding site | 219 | FMN (UniProtKB | ChEBI) | |||
Binding site | 245-246 | FMN (UniProtKB | ChEBI) | |||
Binding site | 267-268 | FMN (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | dihydroorotate dehydrogenase (NAD+) activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
- EC number
- Short namesDHOD B; DHODase B; DHOdehase B
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfuromonadia > Geobacterales > Geobacteraceae > Geobacter
Accessions
- Primary accessionC6E6N2
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000204306 | 1-305 | Dihydroorotate dehydrogenase B (NAD+), catalytic subunit | ||
Interaction
Subunit
Heterotetramer of 2 PyrK and 2 PyrD type B subunits.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)31,883
- Last updated2009-09-01 v1
- MD5 Checksum7B503B98240A1D4AF4C231112814720A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001661 EMBL· GenBank· DDBJ | ACT17873.1 EMBL· GenBank· DDBJ | Genomic DNA |