C6BV89 · SYI_MARSD

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site571L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site615ATP (UniProtKB | ChEBI)
Binding site909Zn2+ (UniProtKB | ChEBI)
Binding site912Zn2+ (UniProtKB | ChEBI)
Binding site928Zn2+ (UniProtKB | ChEBI)
Binding site931Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • Ordered locus names
      Desal_2004

Organism names

Accessions

  • Primary accession
    C6BV89

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10002162331-937Isoleucine--tRNA ligase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif58-68'HIGH' region
Motif612-616'KMSKS' region

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    937
  • Mass (Da)
    107,288
  • Last updated
    2009-09-01 v1
  • Checksum
    00FEF119D2DC0E33
MSDYKKTLCLPKTKFPMKANLKQREPEMLKRWEETGVYEKMVEANAEAEQYVLHDGPPYANGHIHMGTAMNKVLKDIIVKSRNLQGMKAEYVPGWDCHGLPIEHKVEQELKKKKKELPTTVIRKLCREYAAKFVDIQRKEFKRLGVLGNWEDPYLTMKPEYEAATARELGRFMEKGSVIRGKKPIHWCCDCRTALAEAEVEYEDHTSPSIYVRFPLNDEKVLKALPEDVASKVDLSRTYVCIWTTTPWTIPDNMAVAVHPEFEYAVTEVNGDIYILAERLLPVCAESFGWESWNVLATVAGAKLEGAVAKHPIYDRKSPVVLADYVTLDSGTGCVHTAPGHGREDFETGLKNGLEVYSPMNNSGVFLKEVEFFAGMNVWEANPKVIEKLEEVGNLLAQEKISHSYPHCWRCKEPVIFRATTQWFIGMEENDLRKKALKAIKDDVEWLPAWGENRIYSMVENRPDWCISRQRNWGVPIIALICEDCDEVYNDPEWVFSVVDEFEKHERGCDYWFEKSIEEIVPEGLKCPKCGGNHWTKEDDILDVWFDSGTSFAAVVEKRKEHRFPADLYLEGSDQHRGWFHSSLLASVGTRDVPPYKTVLTHGYVVDKDGRKMSKSIGNVIAPQEIIDQHGAEILRMWVSAVNYQEDVRISDEILSRMVDTYRRVRNTCRYILGNLDGFNPETDAVAPADMLPIDHFALDLVNRQHEVIQKAYTNFEFHKVYHTLHNLCTTELSAFYLDIIKDRLYVSGEKSLERRSAQTVLWQTMLMLLKDMAPILSFTAEEAYSHMPEEMKGSAETVFAIRPELLKPSIDDAERKRWELLMDVRTEVTKAIEPLRREKVIGHSLDTKITLFANEEIAKALEGIERREFFIVSGVEIKPLAEASDDAVKPEELEGLSIKVEKAQGEKCSRCWRYDTLGTNAEHPELCPRCAAVLAG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001649
EMBL· GenBank· DDBJ
ACS80064.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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