C6A0I0 · PURL_THESM

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site32
Binding site35ATP (UniProtKB | ChEBI)
Binding site76Mg2+ 1 (UniProtKB | ChEBI)
Binding site77-80substrate
Active site78Proton acceptor
Binding site99substrate
Binding site100Mg2+ 2 (UniProtKB | ChEBI)
Binding site224substrate
Binding site252Mg2+ 2 (UniProtKB | ChEBI)
Binding site296-298substrate
Binding site471ATP (UniProtKB | ChEBI)
Binding site508ATP (UniProtKB | ChEBI)
Binding site509Mg2+ 1 (UniProtKB | ChEBI)
Binding site511substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • Ordered locus names
      TSIB_0056

Organism names

Accessions

  • Primary accession
    C6A0I0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10002060511-713Phosphoribosylformylglycinamidine synthase subunit PurL

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    713
  • Mass (Da)
    78,634
  • Last updated
    2009-09-01 v1
  • Checksum
    07A6FE5934FA4F91
MFPHEEKLIKERLGREPNEVEKAMLEVMWSEHASYKSSRKWLKLLPTENEHVILGPGEDAGVVKFDDNTAIVVGIESHNHPSAVEPYGGAATGVGGIVRDILCMGARPIALLDPIRFGPLGKERNRYLFEYVVKGIADYGNRIGVPTVGGETEFDESLDNYTLVNVACIGLMRPEELVHSYVEESGLLLVLVGNKTGRDGIHGVTFASEELSENAEEEDRSAVQIPDPFTEKLLIEATLEAVHTGKVKALKDLGGGGLTCASSEMAGKKGFGAVIYADRVPQREPNMNPMEIMISESQERMLFAVRKEDLNEITKIFEKYDLEWTVVGETIEEPRYIVYWNDEKIADLPIDLLADVPIIEWEAKSYDIEKDVQTPEISIEEALLEVLSSPNIVSKAWIWQQYDHEVQGRTVLKPGLDATVLKINDEHGLAFVSDGNPSYSHLNPYHGAMSAVAEVVRNLASVGARPLALVDNLNFASPERPEVYWSFIETIKGLADAAKAFGLAYVSGNVSFYNEVGKKPIKPTPVVAGLGKVKLENITTMDFKDKGDLIAVVGTTKKELGGSELYRIFGINKGIAPRVDLEREKQNVEGILKAIELKLVKAVHDVSKGGLAIALIEMAISGNKGFEVDIEKVPAEGGLSPLEVLFSESHGRFLISFEEKNLEKIKAIFDEFAVIGRVAEEMLIFKHENKEVINSDLRQVKALYNSLPSILGE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001463
EMBL· GenBank· DDBJ
ACS89125.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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