C5YAA9 · C5YAA9_SORBI
- ProteinATP-dependent 6-phosphofructokinase
- GenePFK
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids484 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 125 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 190-191 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RG | ||||||
Binding site | 215-218 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GDGT | ||||||
Binding site | 216 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Site | 217 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 244-246 | substrate | ||||
Sequence: TVD | ||||||
Active site | 246 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 289-291 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 345 | substrate | ||||
Sequence: E | ||||||
Binding site | 403-406 | substrate | ||||
Sequence: YMIR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Sorghinae > Sorghum
Accessions
- Primary accessionC5YAA9
Proteomes
Genome annotation databases
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-33 | Disordered | ||||
Sequence: MDPTAARSSGGTPAAAENDATAPTNTTVTLPPL | ||||||
Compositional bias | 14-32 | Polar residues | ||||
Sequence: AAAENDATAPTNTTVTLPP | ||||||
Domain | 118-427 | Phosphofructokinase | ||||
Sequence: AALVTCGGLCPGLNTVLRELVVGLHELYGVRHVFGVAAGYRGFYGTDEDHVRLDPAAVDDWHKKGGTVLKTTRGGFDLGKIVDGIVARGYTQIYAIGGDGTMRGAVAIFQEFKRRGLNISITGIPKTVDNDIGIIDRSFGFQTAVEIAQQAIDAAHVEAVSAVNGVGLVKLMGRSTGHIALHATLSSRDVDCCLIPEVDFHLEGKGGLFEFLYERIKKKGHAVIVVAEGAGQELIPRTDDQKREQDESGNIVFLDVGPWLKSELGRWWKREHPDELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIH |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length484
- Mass (Da)51,972
- Last updated2009-09-01 v1
- Checksum078F61B4B948AC1F
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 14-32 | Polar residues | ||||
Sequence: AAAENDATAPTNTTVTLPP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000765 EMBL· GenBank· DDBJ | EES12338.1 EMBL· GenBank· DDBJ | Genomic DNA |