C5P798 · C5P798_COCP7

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site42-47FMN (UniProtKB | ChEBI)
Binding site89-92FMN (UniProtKB | ChEBI)
Binding site127-136FMN (UniProtKB | ChEBI)
Binding site162FMN (UniProtKB | ChEBI)
Binding site409FAD (UniProtKB | ChEBI)
Binding site439-442FAD (UniProtKB | ChEBI)
Binding site478-481FAD (UniProtKB | ChEBI)
Binding site520NADP+ (UniProtKB | ChEBI)
Binding site589-590NADP+ (UniProtKB | ChEBI)
Binding site595-599NADP+ (UniProtKB | ChEBI)
Binding site669FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      TAH18
    • ORF names
      CPC735_026340

Organism names

Accessions

  • Primary accession
    C5P798

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Mitochondrion
Note: Relocalizes to mitochondria after H2O2 exposure.

Keywords

Interaction

Subunit

Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain36-180Flavodoxin-like
Region211-235Disordered
Compositional bias216-235Polar residues
Domain257-505FAD-binding FR-type

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    669
  • Mass (Da)
    75,551
  • Last updated
    2009-09-01 v1
  • Checksum
    4DA8E8048C31E731
MESRFIYCGALELKRGAFTDEIDEMASSKNGVPRTALVVYASETGNSQEIAEELGRLTERLHFETHVSELDAIEAESLNGHSLTIFAVSTTGQGDVPASGKTFWRSLLLKRLLPTYLQHVNFALFGLGDSSYPKFNWAARKMHKRLLQLGANEIFPSGEADEQHPEGIDGTFVPWAQSLKKFLLDKFPLKPGQHPIPDDVRLPPKWALAPWGQGTGKEHQMTNGNSSQPEKNNGTGKEQIVQYPALQPRDHDTRPIPNSITATLIDNIRVTPESHWQDVRHLTLAVPESIQYIPGDILHITPKNFAKDVNTLLSLMGWEADADIPLCFTPASKSSLSSSYPPISFLQNSPGFTLRELLTNYLDIMAIPRRSFFSQISHFTDDTMQKERLLEFTNPEYIDEYYDYATRSRRSILEVLYEFDTVKVPWQQVCNVFPILRGRQFSIASGGRLKKTVEGKTKFELLVAIVKYQTVIKKIREGVCTRYLAVLQPGSTMKVQLHRGGLSPSVKQLLEPSVVIGPGTGVAPIRSLLWEKAALVEAYRNKHGPNVPSPVGPVILLYGGRNRAADFFFQEEWNKLKETLDLTVFTAFSRDQKHKFYVQDAIRQNKDAFFRVLHDMQGAVFICGSSGRMPQAVREALIETFETRGSSREEAEKYLIDMERVGRYKQETW

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias216-235Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ACFW01000025
EMBL· GenBank· DDBJ
EER27298.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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