C5MFL7 · C5MFL7_CANTT

Function

function

Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

1718100200300400500600700
TypeIDPosition(s)Description
Active site226Proton donor

GO annotations

AspectTerm
Cellular Componentchromosome, telomeric repeat region
Cellular ComponentMre11 complex
Cellular Componentsite of double-strand break
Molecular Function3'-5'-DNA exonuclease activity
Molecular Functionadenylate kinase activity
Molecular Functiondouble-stranded telomeric DNA binding
Molecular FunctionG-quadruplex DNA binding
Molecular Functionmanganese ion binding
Molecular Functionmolecular adaptor activity
Molecular Functionsingle-stranded DNA endodeoxyribonuclease activity
Molecular Functionsingle-stranded telomeric DNA binding
Biological Processascospore formation
Biological Processbase-excision repair
Biological ProcessDNA double-strand break processing involved in repair via synthesis-dependent strand annealing
Biological Processdouble-strand break repair involved in meiotic recombination
Biological Processdouble-strand break repair via break-induced replication
Biological Processdouble-strand break repair via nonhomologous end joining
Biological Processmaintenance of DNA trinucleotide repeats
Biological Processmeiotic DNA double-strand break formation involved in reciprocal meiotic recombination
Biological Processmitochondrial double-strand break repair via homologous recombination
Biological Processmitotic G2 DNA damage checkpoint signaling
Biological Processmitotic intra-S DNA damage checkpoint signaling
Biological ProcessR-loop processing
Biological Processregulation of transcription by RNA polymerase II
Biological Processtelomere maintenance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Double-strand break repair protein

Gene names

    • ORF names
      CTRG_04860

Organism names

Accessions

  • Primary accession
    C5MFL7

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain391-567Mre11 DNA-binding
Region628-718Disordered
Compositional bias632-691Basic and acidic residues

Sequence similarities

Belongs to the MRE11/RAD32 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    718
  • Mass (Da)
    81,248
  • Last updated
    2009-07-28 v1
  • Checksum
    FEDC4828ACAFA0A9
MICLKSNRRSALFFSVSIDEDVTTLTMTGICFCGERKFARDRTNGAGGRQGGREKIKIYHHRQDQTKKKKNATSVIPLVCSCDGFSFISPQREIATFVMPLVDHIEPGPDTIRVLLATDNHVGVYENDPIRGDDAWKTFEEITQLAKQQDVDMIIHGGDLFHINKPSKKSMYHVIKSLRSNCMGDRPCELELLSDPSYLANGVEEINYEDPNLNISVPVFAISGNHDDATGEEFISAIDLLAVTGLINHFGKVRDNDEITVSPVLLQKGTTKLSLYGMSSIRDERLHRLFRDGSVKFQRPSLQTNDWFNFLAFHQNRTEHSYISTIPENFLPNFIDFILWGHEHECIPHPQHNAETKFDVLQGGSSVATQLTEGESAPKHVYVMNIKGKNYSIEAIELKTVRPFVIKDIELSKTDLIPGAASRSDVIAYLTEEAEKAIEEANRVFKEKNGHLFTGESESKLPLPLVRLRVEYSGGFEIENVTRFSNRFVGKVANVNDVVQFYKRKVPPRQKTSLTKKTKFDADLLEEKLSERKANDLKLKDIVSDMLKQTQLTLVPEDGINEAVQKTIDNEDKNALSQFVTREIKRETKALLSIDIDESEFHGSGEKHAKSSFKQFLSQIKELSNPIDLDSINRDLESEPSKPKSKNTRKKPASKSKEMILSSDDSDIEMFVTEDTRRGKSSRKAKSSKPSYAEDDIQILSDEDEYVPPKKSTGRRRR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias632-691Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GG692401
EMBL· GenBank· DDBJ
EER31130.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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