C4YUC4 · C4YUC4_9RICK

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site31Interaction with DNA
Site142Interaction with DNA
Site143Interaction with DNA
Site146Interaction with DNA
Site158Interaction with DNA
Active site304O-(5'-phospho-DNA)-tyrosine intermediate
Site306Interaction with DNA
Site499Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      REIS_1025

Organism names

  • Taxonomic identifier
  • Strain
    • endosymbiont of Ixodes scapularis
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rickettsiales > Rickettsiaceae > Rickettsieae > Rickettsia > spotted fever group

Accessions

  • Primary accession
    C4YUC4

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-111Toprim
Region166-171Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    770
  • Mass (Da)
    87,488
  • Last updated
    2009-07-28 v1
  • Checksum
    764A3E0C4C3A1123
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNKVKSDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKDAKDLTEKLKSQNFYVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKSYGDKYLPTSPRIYKSKVKNVQEAHEAIRPTNITYTPDSLKEKLEKDYYKLYELIWKRTIACQMENVIMDLVVANLASENKEYLAKANGSTIAFDGFYKVCRESVDDEAEEENKMLPPLKEQEPLKTKEVIPNQHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVSLEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKAALSNFWSGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKVCPSCKTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNNENEGEPSAIPNENKVLGTNKDGVEIYLKKGPYGPYIQLGEQEGKVKPKRSPVPANLNQNDITLDIALKLLSLPLKIGVPKDSGEEIMIGYGKFGPYIKYMGKFISVPKKYDFLNLRLDDAMKLIEDNKRS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000770
EMBL· GenBank· DDBJ
EER21844.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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